| The goal of bone tissue engineering is to find a better solution to the problem of large segmental bone defects,bone delayed healing or nonunion.As more and more scholars recognize that the periosteum plays an important role in the process of bone injury repair,the current tissue engineering periosteum is constantly being developed.Researchers have incorporated the concept of tissue engineering into the tissue engineering periosteum,and have prepared materials,cells and cytokines for the three elements of tissue engineering films.It is desirable to be able to simulate the natural periosteum from the structure or function and to use it to promote the repair of bone lesions.The cells extracted from the periosteum and known by the majority of scholars as periosteum derived stem cell(periosteum derived stem cell PDSC)have been shown to express mesenchymal stem cell markers on their surface and have the potential for osteogenesis,cartilage and adipogenic differentiation.So PDSC may be an excellent seed cell for tissue engineering periosteum.There is still a key problem in tissue engineering based on seed cells,that is,the osteogenic ability of seed cells in the process of in vitro expansion is gradually decreased,which may be related to the degeneration of osteogenic progenitor cells,and in the process of cell transplantation,Providing key factors is expected to solve this problem.Fibroblast growth factor-2(FGF-2)was significantly increased in the early healing of fractures,but was low in the subsequent bone formation stage,whereas bone morphogenetic protein-2(BMP-2)in the osteogenic differentiation stage expression.This paper was prepared by a biomimetic release of FGF-2 and BMP-2 chitosan-collagen scaffold,and combined with PDSC,the experiment on repair of calvarial bone defect,proved that PDSC has osteogenic differentiation ability in vitro and in vivo and the bionic controlled-release membrane material can support PDSC proliferation in vivo,and promote bone formation the new bone formation.The main method used in this subject is as follows:1.Preparation of collagen-chitosan scaffolds and biomimetic controlled release of FGF-2 and BMP-2 microcapsules by conventional methods,followed by lyophilization.2.PDSC were extracted and their surface stem cell markers were detected by flow cytometry.Three-line induced differentiation experiments were performed to identify their pluripotency.PDSC was co-cultured with collagen-chitosan scaffold to detect the adhesion,proliferation and differentiation of cells on the scaffold.3.Tissue engineering membrane scaffolds were made,skull defects were transplanted and transplanted.MicroCT and histological examination were used to evaluate the new bone formation.The survival of PDSC was detected by EdU method.4.Statistical analysis:SPSS 20.0 statistical software was used for data analysis.The values of each group were expressed by mean ± SD.One-way ANOVA was used for comparison between groups.Using the LSD test if the variance was equal,in contrast using the Dunnett T3 test for two comparison.A P-value of less than 0.05 was considered statistically significant.Result:1.The experimental PDSC was identified by flow cytometry,and its surface expressed mesenchymal stem cell markers,and induced by the osteogenesis,cartilage,adipogenic and other multi-directional differentiation,proved that the tissue engineering membrane scaffold with proliferation,Osteogenic differentiation.2.MicroCT and histological examination showed that PDSC combined with bionic release of tissue engineering membrane with better bone defect repair ability.Conclusion:The periosteum derived stem cells will be an excellent seed cell of tissue-engineered periosteum,and the sequential release of FGF-2 and BMP-2 will provide a better proliferative and differentiation environment for PDSC,thus improving the bone repair efficiency of bionic tissue engineering periosteum. |
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