The Study Of Preparation And Property Of HAPw/nmZnO-nmCaO Antibacterial Bone Repair Materials

[Objective]Nano zinc oxide(nmZnO)and nano calcium oxide(nmCaO)were used to modify the hydroxyapatite whisker(HAPw),we maked biological ceramic composite materials with antibacterial properties and biological activities,and through design the orthogonal experiment to investigate the best modification process;The technological conditions of preparing porous granular antibacterial bone scaffold by HAPw/nmZnO-nmCaO powder were studied.To investigate the in vitro degradation behavior of porous HAPw/nmZnO-nmCaO scaffold preliminarily.[Materials and Methods]? By used zinc nitrate and calcium nitrate as raw materials,PEG as dispersant,to modify HAPw with sol-gel method,maked HAPw/nmZnO-nmCaO composite materials by high temperature melting attached.Analysis material phase and surface morphology by X-ray diffraction(XRD)and transmission electron microscopy(TEM),and used the method of X-ray energy dispersive spectroscopy(EDX)to analysis the element of the composite materials;To investigate the effect of different reaction temperature on the morphology of HAPw/nmZnO-nmCaO.The effect of pH value on the morphology of HAPw/nmZnO-nmCaO was studied.? The reaction temperature of silica sol and HAPw/nmZnO-nmCaO composite material was determined by XRD at different temperatures;Used HAPw/nmZnO-nmCaO powder as raw material,silica sol as binder,add the control particle size of about 150-250um PMMA,through the mold compression,high temperature calcination,grinding and sieving to obtain porous granular bone scaffold,applied the test of XRD,scanning electron microscope,universal testing machine and Archimedes drainage method to analysis material phase,surface morphology,mechanical behavior and porosity,respectively;.?The porous HAPw/nmZnO-nmCaO antibacterial bone scaffold material was placed into the citric acid buffer to observe its degradation in the extreme environment.[Result]Under the optimal modification process,the characteristic peaks of the calcium oxide and Zinc Oxide were also found on the diffraction pattern of the modified materials,and the peak of the main phase hydroxyapatite was not affected.Under the transmission electron microscope,rnmZnO particles and nmCaO particles were attached to the surface of HAPw at the same time uniformly,the nmCaO particles were round granular,and the nmZnO particles were six square shape,particles and whiskers are bonded by chemical bonds.With the change of the calcination temperature,when the temperature is higher than 600 ?,the XRD spectrum showed the impurity peaks such as calcium silicate and zinc silicate.When the weight of the pore forming agent is 25%,the porosity is 63%,and the compressive strength is 2.6MPa.The XRD spectra showed that the SiO2 characteristic peaks were found in the porous scaffolds except for the characteristic peaks of main phase HAPw,ZnO,and CaO.The results of SEM showed that when join the PMMA as pore forming agent to make the porous HAPw/nmZnO-nmCaO antibacterial bone scaffold material,the pore size was range from 150-250um.The morphology of nmZnO and nmCaO did not change;The degradation rate of porous HAPw/nmZnO-nmCaO antibacterial bone scaffold material and porous HAPw in citrate buffer after 120h was 29.37%and 15.27%.,respectively.[Conclusion]With calcium nitrate and zinc nitrate as raw materials,the hydroxyapatite whisker was modified by sol-gel method,the optimal pH was 6.2 and 6.4.,the optimal temperature was 70?.Different pH value,temperature and weight of PEG6000 could form different morphologies of HAPw/nmZnO-nmCaO;By adding silica sol as binder to make porous HAPw/nmZnO-nmCaO antibacterial bone scaffold material,the calcination temperature could not exceed 600?;Used PMMA as pore forming agent and silica gel as binder,the porous granular HAPw/nmZnO-nmCaO antibacterial bone scaffold material could be prepared by high pressure sintering,high temperature calcination and sieving,the pore size was between 150-250um,the best porosity and compressive strength was 63%and 2.6MPa,respectively.Porous granular HAPw/nmZnO-nmCaO antibacterial bone scaffold material could be degraded by soaking in citric acid buffer.