Changes And Value Of Fecal SIgA And Intestinal Barrier Function In Children With Different Type Of Henoch-Schonlein Purpura

Objectives:To detect sIgA in feces and diamine oxidase, D-lactates and endotoxin in plasma in children with Henoch-Schonlein purpura(HSP) and to explore their intestinal mucosal immunity and intestinal barrier function in order to understand further the pathogenesis of HSP and to provide evidence for the treatment.Methods: The children with HSP at acute and recovery phase in department of pediatrics of the first affiliated hospital of kunming medical university during and healthy children volunteer from the school were enrolled in our study. Their blood and stools are collected. The contents of sIgA in the feces and the D-lactate and endotoxin in the isolated plasma were detected by enzyme linked immunosorbent assay(ELISA). The content of the diamine oxidase in the isolated plasma were detected by spectrophotography.Results: The level of sIgA in the feces is significantly higher in children with HSP at acute and recovery stage than in healthy children group(P < 0.017) , but there were not different between acute and recovery stage(P> 0.017).The level in abdominal type of HSP at acute and recovery stage is significantly higher than in healthy children group(P < 0.017), but there were not different between non abdominal type group and healthy children group and between abdominal type and non abdominal type of HSP(P >0.017).The level of the diamine oxidase in plasma is significantly higher in children with HSP at acute and recovery stage than in healthy children group(P< 0.05) , but there were not different between acute and recovery stage (P > 0.05); The level is significantly higher in abdominal type and non abdominal type of HSP at acute and recovery stage than in healthy children group(P < 0.05),but there were not different between abdominal type and non abdominal type at acute and recovery stage (P>0.05).The level of the D-lactate in plasma is significantly higher in children with HSP at acute stage than in healthy children group(P < 0.017) , but there were not different between recovery stage and healthy children group, and between acute and recovery stage (P>0.017); The level is significantly higher in abdominal type of HSP at acute stage than in healthy children group(P < 0.017), but there were not different between non abdominal type group and healthy children group, and between abdominal type and non abdominal type of HSP at acute stage(P > 0.017); there were not different among abdominal type of HSP at recovery stage, non abdominal type of HSP and healthy children (P > 0.05).The level of endotoxin in plasma is significantly higher in children with HSP at acute and recovery stage than in healthy children group(P< 0.017),but there were not different between acute and recovery stage(P> 0.017); The level is significantly higher in abdominal type of HSP at acute and recovery stage than in healthy children group(P < 0.017), but there were not different between non abdominal type group and healthy children group, and between abdominal type and non abdominal type of HSP(P> 0.017).Conclusions: The levels of the diamine oxidase, D-lactates and endotoxin in plasma in children with HSP is in increase, it is suggested that the intestinal permeability is increased and the intestinal barrier is dysfunction; The level of sIgA in feces in children with HSP is in increase reactively, it is maybe because of the intestinal barrier dysfunction; The intestinal barrier dysfunction are still not recovery in children with HSP at recovery stage; The intestinal barrier both in abdominal type and non-abdominal type in children with HSP is dysfunction, but more serious in abdominal type of HSP than in non-abdominal type of HSP.