Determination Of Residual Chloroform In Medical Polylactic Acid Membrane By Gas Chromatography

Objective: To establish a method to determine the residual chloroform in the medical polylactic acid membrane.Methods: The direct aqueous injection gas chromatographic method was established for the determination by using GC Solution workstation software and RESTEK-RTX capillary-column chromatography（Shimadzu Japan,30m×0.25 mm×0.25 μm）.The detector was electron capture detector（Electron capture detector,ECD）,and the detector temperature was 270 ℃.The injector temperature was 200 ℃.The column temperature was 85 ℃,maintained for 3min.The high purity nitrogen was used as the carrier gas with the flow of 1.0ml·min^-1.The split ratio of sampling was 10:1.The injection volume was 1μl.Results: The calibration curve showed a good linearity within the range of25-2000 ng·ml^-1（r=0.9999）.The limit of detection was 1.50 ng·ml^-1,and the limit of quantitation was 4.62 ng·ml^-1.The average recovery rate was 98.35%,RSD=1.98%.Conclusion: The method is sensitive and accurate,which can be used for determining the residual chloroform in the medical polylactic acid membrane.