Effect Of Paclitaxel Delivery System Based On Chitosan Hollow Nanospheres Toward Human Lung Cancer A549 Cells

ObjectiveIn order to increase the solubility of poorly water-soluble drugs and to adjust its release and to improve its efficacy,we prepared biodegradable chitosan hollow nanospheres(CHN)for loading paclitaxel(PTX)and studied the effect of paclitaxel delivery system based on chitosan hollow nanospheres(PTX-CHN)toward human lung cancer A549 cells.MethodsIn this study,The morphology of CHN were investigated by Transmission electron microscopy(TEM)and scanning electron microscopy(SEM)and the paclitaxel(PTX),as a model drug,was loaded into CHN by the adsorption equilibrium method.The solid state characterization of PTX was investigated by differential scanning calorimetry(DSC),powder X-ray diffraction(PXRD),and Fourier transform infrared spectroscopy method(FTIR),respectively.The in vitro release experiments investigated the release behavior of PTX.Cytotoxicity assay and cell uptake experiments explored the effect of the administration system on A549 cells.The In vivo pharmacokinetics assay studied the bioavailbility of PTX-CHN.ResultsWe have successfully fabricated the biodegradable CHN with good efficacy.The PTX-CHN offered sustained PTX release.The state characterization of PTX by DSC,PXRD and FTIR showed that the PTX absorbed into CHN existed in an amorphous state.An in vitro toxicity experiment indicated that the PTX-CHN produced a marked inhibition for proliferation of A549 cells and promoted apoptosis.A cell uptake experiment indicated that PTX-CHN was successfully taken up by lung cancer A549 cells.Furthermore,a degradation experiment revealed that CHN were biodegradable.ConclusionsBiodegradable CHN can increase the solubility,adjust release,promote absorption of PTX and improve efficacy.It was a promising biomaterials for lung cancer treatment.