The Study Of Nanocellulose Nanocrystal And Its Derivatives As Drug Slow Release Meterial

Nanocrystalline cellulose(NCC)is obtained by hydrolysis of microcrystalline cellulose with acid.NCC is biocompatibility,biodegradability,environmental friendly and friendly renewable biological macromolecular materials.Because of its superior performance,NCC has been paid to its application in the medical field.From excipient of metal nanoparticles to its application as carriers,NCC shows its good performance as nano-materials carriers.Due to the specific surface area of NCC is larger,stronger hydrogen bonding interaction of surface of NCC caused gather between molecules.In order to break NCC aggregate phenomenon respectively,chemical modification was conducted for NCC.The hydroxyl of NCC surface was derived into carboxymethyl and amino to get carboxymethyl nanocrystalline cellulose(N-CMC)and quaternary ammoniation nanocrystalline cellulose(N-QC)to abate aggregate phenomenon of NCC.The purpose of this paper is to the preliminary research and discussion for NCC,N-CMC and N-QC as slow release drug delivery material.The model drug is EN-9(Tyr-Pro-Phe-Phe-Gln-Pro-Gln-Arg-Phe-NH2)which was synthesized by our term.EN-9 has high analgesic activity,low addiction and low tolerance of chimeric peptide.This paper preliminary studies EN-9-NCC,EN-9-N-CMC and EN-9-N-QC nanoparticles preparation to determine the EN-9-NCC,EN-9N-CMC and EN-9N-QC nanoparticles prepare conditions that is:at room temperature,200 r min-1 of stirring speed,EN-9 and NCC(CMC,QC)in deionized water and were incubated for 48 h and encapsulation efficiency were 84.7%,72.37%and 95.12%.Release test of EN-9-NCC,EN-9-N-CMC and EN-9-N-QC nanoparticles in vitro respectively were detected in simulated gastric juice,intestinal juice,physiological fluids and deionized water.Explosive release was before 1.5 h and after 1.5 h time it is slow release to 4 h after close to balance,release rate is over 75%.Analgesic activity experiment showed that EN-9-NCC,EN-9-N-CMC and EN-9-N-QC nanoparticles have analgesic activity in vivo.Analgesia activities of EN-9-NCC,EN-9-N-CMC and EN-9-N-QC nanoparticles were weaker than that of EN-9,but the analgesic times of EN-9-NCC,EN-9-N-CMC and EN-9-N-QC nanoparticles were much longer than that of EN-9.The analgesic time and strength of EN-9-N-QC nanoparticles was better than that of EN-9-NCC and EN-9-N-CMC nanoparticles.This research provided referential basis for the new preparation formulation of EN-9 and the application of NCC and its derivatives as long-time release carrier.