Studies On Rosemary Extract Attenuates Oxidative-stress In HepG2 Cell

Rosemary (Rosmarinus officinalis L.), also known as the Labiatae rosemary perennial evergreen shrub plant, which contains carnosic acid, rosmarinic acid, rosmanol,rosmariquinone, caffeic acid, Rosemary phenol, ursolic acid and other active ingredients.Rosemary has obvious antioxidant, anti-tumor, anti-inflammatory, antibacterial and other physiological activities, that is rich in medicinal value and edible value. In this paper,HepG2 were choosed as experimental model organisms. The antioxidant activity in vivo was studied in order to evaluate the antioxidant capacity and the molecular mechanism of its action of rosemary extract (RE). The study of this paper will provide some guidance for the scientific development and nutrition and dietary intervention of rosemary resources.In this paper, the effect of RE on the viability of HepG2 cells was detected by MTT assay, while the effect of different concentrations of H2O2 on the cell damage was also detected. After the appropriate concentration of RE cells was choosed, the survival rate of HepG2 injured by H2O2 was also investigated. 2',7' -Dichlorofluorescin (DCFHDA) and monochlorobimane (MCB) was used to detect the ROS and GSH in cells by laser scanningconfocal microscope respectiveiy. Comet assay was used to detect the damage level of DNA in nucleus, and the expression level of mRNA was used by RT-PCR.It was found that RE can significantly alleviate the oxidative damage induced by H2O2 cells and decrease the content of ROS of damaged cells while increase the content of GSH.On the other hand, RE could inhibit the damage of DNA induced by H2O2, and adjust the expression levels of antioxidant genes.After RE was storaged in the high temperature conditions with different time, LC was used to detect changes of its components. Then the antioxidant activites were detected by DPPH method, the effect of different processing time RE on the survival rate of HepG2 cells injured by H2O2 was method on MTT assay too, and the content of ROS, GSH was also be explored.The results show that RE can significantly alleviate the oxidative damage of HepG2 cells induced by H2O2, regulate the expression level of mRNA gene of endogenous antioxidant enzyme, increase GSH content whlie inhibited the accumulation of oxidation products and improve the ability of model organism to scavenge free radicals. In addition,after processing, carnosic acid content was decreased significantly while carnosol content was increased in RE. The cell experiments demonstrated that the antioxidation of RE extract after processing was decreased, and it was associated with the content of carnosic acid in RE.