Clinical Study Of Serum Ox-LDL,hs-CRP,MMP-9 Levels And Carotid Atherosclerotic Plaques In Atherosclerotic Cerebral Infarction

Objective: to detect the serum oxidized low density lipoprotein(ox-LDL)and matrix metalloproteinase-9(MMP-9)and high sensitive C reactive protein(hs-CRP)expression in acute atherosclerotic cerebral infarction,combined with carotid artery ultrasonography,observation of carotid artery in patients with cerebral infarction hard plaque and stenosis of acute coronary atherosclerosis.To investigate the serum levels of oxidized low density lipoprotein(ox-LDL)and matrix metalloproteinase-9(MMP-9)and high sensitive C reactive protein(hs-CRP)associated with acute atherosclerotic cerebral infarction patients with carotid artery plaque stability and recurrence of cerebral infarction risk stratification,to further clarify the acute atherosclerotic cerebral infarction risk factors of early warning,and serum oxidized low density lipoprotein(ox-LDL)and matrix metalloproteinase-9(MMP-9)and high sensitive C reactive protein(hs-CRP)as a biomarker of vulnerable plaque screening in clinical practice,the risk for recurrence of atherosclerotic cerebral infarction prediction,assessing disease severity and effective treatment and prognosis.Method:1 By October 2015 to August 2016 in patients with atherosclerotic cerebral infarction neurology department of First Affiliated Hospital of Hebei North University and the incidence within 7 days of the 100 cases of cerebral infarction group,healthy subjects(artery by color ultrasound diagnosed,without carotid atherosclerotic plaque and stenosis)of 50 patients in the control group,the carotid artery(CCA)distal(carotid enlargement Department of proximal)at 1cm(1 vessel segments),bifurcation(Bulb)(length 1cm)at(2 vessels),the bifurcation of the distal internal carotid artery(ICA)at 1cm(3 vessel segments),a total length of about 3cm of the carotid artery within the range of color ultrasonography.2 Carotid IMT and plaque judgment: usually IMT <1.0mm as a marker of carotid artery ultrasound negative,when IMT is greater than or equal to 1.0mm and less than1.5mm,and accompanied by endometrial echo discontinuity,but not protruding into the lumen,called IMT thickening.The plaques are defined as IMT > 1.5mm from the interface between the intima and lumen to the interface between the central membrane and the outer membrane,and the local structure changes and is convex to the lumen.The carotid ultrasound examination included 3 vessels in each side and 6 vessels in both sides.At the same time,according to the standard,the patients received fasting elbow vein blood 3ml in the morning of next hospital,and the physical examination also took the morning fasting elbow vein blood 3ml.After centrifugation,take serum 0.5ml and keep it in-80 centigrade refrigerator for testing.The concentrations of serum ox-LDL,MMP-9 and hs-CRP were determined by enzyme labeled immunosorbent assay(Enzyme,linked,immunosorbent,assay,ELISA).3 ESRS assessed on cerebral infarction subjects,ESRS more than 4 cent is defined as high risk of recurrence of cerebral infarction group,ESRS < 4 divided into low-risk group the recurrence of cerebral infarction.The concentrations of serum ox-LDL,MMP-9,hs-CRP,and carotid plaques in two recurrence risk groups were observed.4 Simultaneous determination of blood pressure,blood glucose,smoking,height,weight and other aspects of the patients,and finally analysis of acute cerebral infarction group and health examination group of the above indicators of the difference between the comparison and statistical analysis.Results: the risk of recurrent cerebral infarction group serum ox-LDL,hs-CRP and MMP-9 levels,carotid intima-media thickness and carotid artery plaque detection rate was significantly higher than that of control group,the results showed that the difference was statistically significant(P < 0.05).In addition,Spearman correlation analysis showed that serum ox-LDL,hs-CRP and MMP-9 were positively correlated with the incidence of atherosclerotic cerebral infarction(P<0.05).The serum levels of ox-LDL,hs-CRP and MMP-9 in the non plaque group,the stable plaque group and the unstable plaque group were increased successively in the patients with cerebral infarction,and the difference was statistically significant(P<0.05).Conclusion:1 Increased atherosclerosis and serum levels of ox-LDL,hs-CRP and MMP-9 levels in patients with cerebral infarction,accelerated in patients with carotid artery plaque formation,and promote plaque off,and then become independent risk factors of cerebrovascular disease.2 Biological detection in elderly patients with ox-LDL,hs-CRP and MMP-9 levels can be used to predict the formation of AS risk screening index,and will serve as a high-risk population of this part of the population,the need for further evaluation of atherosclerotic plaque inspection.3 Detection of serum ox-LDL,hs-CRP and MMP-9 is important for early screening and prevention of carotid plaque and atherosclerosis.Carotid atherosclerotic plaques,carotid IMT thickening,plaque formation,and stability can be used as predictors of high risk of recurrent stroke in atherosclerotic cerebral infarction patients.