The Research Of KLF-8 In Placenta Of Preeclampsia Patients

Objective: Preeclampsia is a gestational idiopathic systemic disease,which is a serious complication of pregnancy,but also is one of the important reasons of China’s maternal and perinatal death [1,2].It mainly occurs in the 20 weeks gestation,and the main clinical manifestations were hypertension,proteinuria,edema,and accompanied by multiple organ dysfunction,which is a serious threat to the safety of mother and child.Preeclampsia is one of the leading causes of maternal and perinatal morbidity and mortality,and the basic pathological changes of preeclampsia is mainly systemic small artery spasm,which caused a series of complications.With the further condition it may develop to eclampsia which leading to multiple organ failure.At present,the etiology and pathogenesis of preeclampsia is not yet clear,but with the clinical and epidemiological in-depth exploration,a large number of researchs confirmed that trophoblast invasion ability abnormalities,oxidative stress,immune response and anti-angiogenic response may caused pre-eclampsia.The expression of KLF-8 is closely related to the hypertensive disorder complicating pregnancy.The purpose of this study was to investigate the expression of KLF-8 in placenta of early onset and late onset severe preeclampsia and healthy pregnant women,and to explore the relationship between KLF-8 and preeclampsia.Methods:1 40 cases of severe preeclampsia in pregnant women in cesarean section in the first people’s Hospital of Wuan city from September 2015 to 2016 03 month were collected,which including early-onset severe preeclampsia 20cases(gestational age <34 weeks)and late onset severe preeclampsia 20 cases(incidence of gestational age over 34 weeks).20 cases of control elective cesarean section group selection of full-term healthy pregnant women were collected.All of the subjects selected were first,single fetus and fetalmalformation and the pregnant women who had no history of hypertension,heart disease,diabetes,kidney disease hyperthyroidism,anemia,autoimmune disease.The women collected were no smoking history,alcohol abuse and long-term drug history.The liver and kidney function and coagulation function of the women were normal.The age,menstrual history,reproductive history and family history of the women were recorded in detail.The three groups were compared and the number of cases,age,parity,smoking and drinking,there was no significant difference(P>0.05).2 After informed consent,all the research object of the delivery of the placenta in 20 min,avoid the calcification area and necrotic area see,away from the umbilical cord insertion site,the maternal surface tissue of placenta 3blocks,each about the size of 1cm in diameter,phosphate buffer(PBS)repeated rinsing,put a piece in 4% paraformaldehyde,another 2 pieces placed in the sterilizing enzyme in freezing tube,quickly placed in liquid nitrogen,then put in the save-80 C refrigerator,until after the determination.The expression of KLF-8 protein in placenta tissue was detected by immunohistochemical SP method and the expression of KLF-8 mRNA in placenta was detected by fluorescence quantitative PCR.3 SPSS 21.0 was used for data analysis and the data was normal distribution,the results with the mean ± standard deviation(x ± s)that the comparison between groups using independent samples t test and P <0.05 as a statistically significant difference in the standard.Results:1 The average age of the early onset severe preeclampsia group was26.60 ± 0.91 years old,the age distribution was 26-40 years old,while the average age of the late onset group was 23.90 ± 0.62 years old,the age distribution was 23-33 years old and the average age of the control group was(24.48 ± 0.71)years,the age distribution was 22-34 years old.Age difference was not statistically significant(P> 0.05)(Table 1).2 In the three groups were visible Brown positive staining cells,cytoplasmic and nuclear KLF-8 protein mainly expressed in placentalsyncytiotrophoblast.3 The expression of KLF-8 mRNA could be detected in the three groups of placental tissues.The level of KLF-8mRNA in placenta of early onset severe preeclampsia was 0.65±0.07,and the late type was0.96±0.09 in the control group,and the control group was 0.98±0.06.The level of KLF-8mRNA in placenta of early onset severe preeclampsia was significantly lower than that of late onset group(P<0.01),but there was no significant difference between late onset group and control group(P>0.05)(Table.2,Table.3).4 The expression of KLF-8 protein could be detected in the three groups of placental tissues.The KLF-8 protein level in early onset severe preeclampsia group was 0.61±0.05,and the late onset was 0.95±0.06,and the control group was 0.98±0.07.The early onset group was significantly lower than that of the late-onset group,the difference was statistically significant(P<0.01),but there was no significant difference between the late onset group and the control group(P>0.05)(Table 4,Table 5).Conclusion: The results of this study indicate that the expression of KLF-8 mRNA and protein in placenta of early onset severe preeclampsia is significantly lower than that of late onset group,but there is no significant difference between late onset group and control group.KLF-8 is mainly expressed in trophoblast cells which are closely related to cell invasion,suggesting that the decrease of KLF-8 expression in placental tissues may be related to the decrease of invasiveness of early onset severe preeclampsia.It confirmed that the early onset of severe preeclampsia pathogenesis and late onset may be different.The decrease of KLF-8 protein and mRNA expression in placenta affect the invasion of uterine spiral artery recasting and trophoblast cells,which indicates that KLF-8 is involved in the pathogenesis of early onset severe preeclampsia.