Expression Changes Of PEGFR After Decompression Of Compressed Spinal Cord Injury In Rats

It has been found that the main cause of demyelination following compressed spinal cord injury(CSCI)is oligodendrocytes apoptosis.The data showed that p EGFR can not only promote the proliferation and differentiation of oligodendrocytes,but also inhibit the apoptosis of oligodendrocytes,suggesting that EGFR activation may have a protective effect on neural myelin sheaths.However,the expression of pEGFR after decompression of CSCI was not reported.Objective: To investigate the changes of pEGFR,pAkt1 expression in rat after decompression of compressed spinal cord injury(CSCI),and its correlation with nerve structure and function change,provide experimental basis for the development of therapeutic strategies and the development of medication for the treatments of CSCI after decompression.Methods: The CSCI model was established with a self-made device,undergone spinal decompression.The motor functions were monitored by Basso,Beattie & Bresnahan locomotor rating scale.The pathological changes in axonal myelinated fibers were estimated by luxol fast blue(LFB).Epidermal growth factor receptor(EGFR),phosphorylated Akt1(p Akt1)were detected by double-labeling immunofluorescence and western blotting assays.Results: The motor functions and number of myelinated nerve fibers were increased along with time extending after decompression.The expression of EGFR,pAkt1 was also increased after decompression,which was consistent with the changes of motor functions and number of myelinated nerve fibers.Conclusion: The structure and function of the injured nerve can be restored to a certain extent,and the expression of pEGFR is closely related to this phenomenon after decompression.It suggests that the activation of EGFR is involved in the endogenous repair of CSCI after decompression.