Researches Of Bacteriostatic And Cell Compatibility Of Pure Magnesium Micro-arc Oxidation-Chitosan/sodium Alginate Containing Copper Composite Coating

Objective: to making a biological composite coating by a multi-coating composite technology and the coating made can not only control the degradation speed,but also has good bacteriostatic and cell compatibility in human body.Methods: Different composite coatings,which are pure magnesium micro arc oxidation(MAO)(group A),pure magnesium MAO-chitosan composite(group B),pure magnesium MAO-chitosan/sodium alginate composite coating(group C)and pure magnesium MAOchitosan/alginate containing copper composite coating(group D),are made by micro arc oxidation of pure magnesium,electroplating of chitosan and sodium alginate and chemical plating copper.Microstructures of composite coatings are obtained by scanning electron microscopy(SEM).The wetting angles of the coatings are measured.The materials and mice pre-osteoblasts are co-cultured,the adhesion and proliferation of cells are measured by CCK-8 experiment at the first day,the third day,the fifth day and the seven day.The cells activity is measured by ALP experiments.The density,distribution and the distribution of cytoskeleton proteins of cells are measured by laser confocal detection cell morphology.The materials and Escherichia coli are co-cultured,and after 24 hours their bacteriostatic rates are obtained.Results: The SEM experiment shows that the micropore and crackle decrease gradually in group A,B and C,and micro mesh structure is appeared in the surface of group D.The wetting angle experiment shows that the coatings of group A are obviously hydrophilic,the coatings of group B and C are hydrophobic and the coatings of group D have stronger hadrophilia than group B and C but weaker than A.The CCK-8 and ALP experiments show that the adhesion and proliferation,and cells activity of group A,B and C increase gradually.And group C is similar to group D.The laser confocal detection cell morphology experiment shows that the cells spread area,density and cytoskeleton tension wire of group A,B and C increase gradually.And group C is similar to group D.The bacteriostatic experiment shows that the bacteriostatic rates of group A,B,C and D increase gradually.Conclusions: Pure magnesium micro-arc oxidation-chitosan/sodium alginate containing copper composite coating can promote the mouse osteoblast adhesion,proliferation,growth,differentiation,cell activity and co cultured,indicating that the material has good biocompatibility.Pure magnesium micro-arc oxidation-chitosan/sodium alginate containing copper composite coating has obvious inhibitory effect on the growth of Escherichia coli and it is a kind of antibacterial material.