Correlation Between Interleukin-37 And Coronary Heart Disease And The Study On Its Role In Disease

Objective:In our study,by detecting the expression of IL-37 in PBMCs of patients with coronary heart disease(CHD),as well as the expression of pro-inflammatory cytokines TNF-?,IL-1?,IL-6 and IL-17 in PBMCs with or without stimulated by recombinant IL-37 protein,we have investigated the correlation between IL-37 and CHD and its role in disease.Methods:(1)Cloning,expression and purification of IL-37 protein,and identify the biological activity of IL-37.(2)Peripheral blood samples were collected from CHD patients and healthy controls.PBMCs were isolated,cultured in vitro and stimulated by recombinant IL-37 protein.The expression of IL-37 in PBMCs,as well as the expression of pro-inflammatory cytokines TNF-?,IL-1?,IL-6 and IL-17 in PBMCs with or without stimulated by recombinant IL-37 protein,were determined by real-time polymerase chain reaction(RT-PCR).Results:(1)We successfully cloned,expressed and purified IL-37 protein,and identified its biological activity.(2)Compared to HC group,the IL-37 levels is increased in CHD patients significantly(p<0.01).Among them,AMI patients and UAP patients showed higher levels of IL-37 compared to HC group(p<0.01),while SAP patients have no significant differences(P=0.97).(3)The levels of TNF-??IL-1??IL-6?IL-17 is increased in CHD patients compared to HC group(P<0.05)and obviously attenuated after stimulated by recombinant IL-37 protein(p<0.01).Conclusion:IL-37 as well as the pro-inflammatory cytokines TNF-?,IL-1?,IL-6 and IL-17 are highly expressed in CHD patients,and the recombinant IL-37 protein inhibits the expressions of the pro-inflammatory cytokines,indicating that IL-37 plays an anti-inflammatory role in CHD.