| Objective:To investigate the expression of CD44 and CD133 in gastric carcinoma and its relationship with clinicopathological parameters and provide guidance for clinical treatment and prognosis.Methods: 100 cases of gastric cancer patients underwent surgical treatment from April 2015 to February 2017 in Tianjin Medical University General Hospital were collected.Immunohistochemical method and immunofluorescence double labeling method were used to detect the expression of CD44 and CD133 proteins in gastric cancer tissues.At the same time,the clinical and pathological data of the patients were collected.Chi square test and multivariate Logistic regression model were used to analyze the relationship between the expression of CD44 protein and CD133 protein and clinical pathological parameters of patients.The Spearman rank correlation test was used to analyze whether there was a correlation between CD44 and CD133 expression.The ordered multi class Logistic regression model was used to analyze the relationship between the expression levels of two proteins and the TNM stage of the patients.The clinical significance of the expression of CD44 and CD133 was analyzed retrospectively.Results:(1)CD44 was mainly expressed in the cell membrane of tumor cells,distributed along the basolateral side of the adenocarcinoma or on the common wall of the gland.In 100 cases of gastric cancer,the positive expression rate of CD44 was 45.0%(45/100),30 cases with low expression and 15 cases with high expression.CD133 is mainly expressed in tumor cell cell membrane and glandular tumor cell debris,part of the cytoplasm.In 100 cases of gastric cancer specimens,the positive expression rate of CD133 was 36.0%(36/100).Including low expression of 21 cases,high expression of 15 cases.The positive expression rate of CD44 and CD133 was 24.0%(24/100),and the negative expression rate was 43.0%(43/100).(2)The expression of CD44 in gastric carcinoma was correlated with tumor size,Lauren type,T stage,N stage and TNM stage(P <0.05).But not with the age,sex,tumor location,WHO pathological type,M stage,whether there is vascular invasion(P> 0.05).Logistic multivariate analysis showed that T stage and N staging were independent risk factors affecting the expression of CD44 in gastric cancer tissues.(3)The expression of CD133 in gastric carcinoma was correlated with tumor size,T stage,N stage,TNM stage,and vessel invasion(P <0.05).But not with age,sex,tumor location,WHO pathologic type,Lauren type,M stage(P> 0.05).Logistic multivariate analysis showed that T staging,N staging,TNM staging,and the presence of vascular invasion were independent risk factors for the expression of CD133 in gastric cancer(P <0.05).(4)Spearman rank correlation analysis showed that there was a weak positive correlation between CD44 expression and CD133 expression(r=0.314,p=0.001).(5)The effects of CD44 and CD133 expression on T staging: the CD44 high expression group was used as the reference,and the risk coefficient of negative group was(P < 0.05).However,compared with the high expression group,the expression of the low expression group was 0.868,but the difference was not statistically significant(P > 0.05).With the high expression group of CD133 as the reference,the risk coefficient of negative group was 0.091(P < 0.05).However,in the low expression group,the risk coefficient was 0.816,but the difference was not statistically significant(P < < 0.05).The risk factor of non dual positive patients was 0.050(P=0.000).(6)The influence of CD44 and CD133 expression on N staging: the expression of CD44 was higher than that of group,the risk coefficient of negative group was 0.255,and the risk coefficient of low expression was statistically significant(P < 0.05).With the high expression group of CD133 as the reference,the risk coefficient of negative group was 0.110,and the risk coefficient of low expression group was 0.209,the difference was statistically significant(P < 0.05).The risk factor of non dual positive patients was 0.473,and the difference was statistically significant(P=0.000).(7)The effects of CD44 and CD133 expression on M staging: the difference in the expression level of CD44 and CD133 had no significant effect on M stage(P = 0.05).However,the analysis showed that the M stage of the double-positive group was significantly higher than that of the non double-positive group(P=0.000).(8)The effects of CD44 and CD133 expression on TNM staging: the CD44 highexpression group was used as the reference,and the risk coefficient of negative group was(P < 0.05).However,the expression of CD44 was lower in the low expression group than that in the high expression group,although the risk coefficient was 0.680,but the difference was not statistically significant(P > 0.05).With the high expression group of CD133 as the reference,the risk coefficient of negative group was 0.367(P < 0.05).However,the expression of CD133 was lower in the low expression group than that in the high expression group,although the risk coefficient was 0.414,but the difference was not statistically significant(P > 0.05).The risk factor of non dual positive patients was 0.076,and the difference was statistically significant(P=0.000).Conclusion:(1)CD44 and CD133 were positive in gastric cancer tissues.The positive rate of CD44 was slightly higher than that of CD133.The two groups were mainly expressed in the cell membrane of tumor cells,and a small amount of expression was found in the cytoplasm.(2)The expression of CD44 and CD133 are related to tumor size,depth of invasion,lymph node metastasis and so on,which suggests that the two proteins may play a role in the development and progression of gastric cancer.(3)There may be some mechanism between CD44 and CD133 expression,which promotes and interacts with each other.(4)CD44 and CD133 were closely related to the stage of gastric cancer,and could be used as potential risk factors for the prognosis of patients with gastric cancer. |
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