The Relationship Of Serum Betatrophin With Insulin Resistance And Islet ? Cell Function In People With Different Glucose Tolerance

Objective To detect serum betatrophin levels in people with different glucose tolerance and to investigate the relationship between serum betatrophin levels and insulin secretion in pancreatic ? cells as well as insulin resistance.Methods 47 patients newly diagnosed with type 2 diabetes mellitus(T2DM group),27 patients with impaired glucose regulation(IGR group)and 36 individuals with normal glucose tolerance(NGT group)were recruited in the study.All subjects underwent intravenous glucose tolerance test(IVGTT).Insulin levels during the first 10 min were measured and acute insulin response(AIR3-5),0-10 minutes insulin the area under the curve(AUC)were evaluated.Homeostasis model assessments for insulin resistance(HOMA-IR)and for ?-cell function(HOMA-?)were calculated.Meanwhile fasting serum betatrophin was determined by ELISA.The relationships between serum betatrophin,those indexes mentioned above and metabolic parameters were analyzed.Comparisons between groups were performed with analysis of variance(ANOVA).Pearson correlation analysis was used to determine simple bivariate relationships.Results The levels of serum betatrophin were significantly increased in the T2 DM group and IGR group than those in the NGT group(2.08(1.08-2.89),1.52(0.54-2.55)and 0.76(0.44-0.97)?g/L,respectively,F=16.142,P<0.01).The finding in the correlation analysis was that the serum betatrophin levels in all participants were positively correlated with weight,body mass index(BMI),waist circumference(WC),waist hip ratio(WHR),fasting plasma glucose(FPG),2 h postprandial plasma glucose(2hPG),Hb A1 c,fasting serum insulin(FINS),2 h postprandial serum insulin(INS120),HOMA-IR and triglyceride(TG)(all P<0.05),and negatively related to the high-density lipoprotein-cholesterol(HDL-C)(P<0.01).But there were no correlation between the serum betatrophin and age,AIR3-5,AUC and HOMA-?(P>0.05).Stepwise multiple regression analysis showed that HOMA-IR and TG were independently related to serum betatrophin levels(?HOMA-IR=0.438,P<0.001;?TG =0.300,P=0.002).Conclusions Serum betatrophin levels are increased in patients with T2 DM and IGR,and are closely related with glucolipid metabolism,but have no direct relation with first-phase of glucose-stimulated insulin secretion.