| Objective:1.To research the changes of peripheral blood lymphocyte subsets,CD4+T cell subsets and their correlation with ESR and BDCAF score in patients with BD;2.The influence of low dose IL-2 on Th17/Treg in peripheral blood of patients with BD.Methods:Eighty-nine patients with BD were enrolled in the study(56.18 % female;mean age±SD,34.79±13.88 years),forty age-matched and sex-matched volunteer healthy donors were also included as control group(62.50% female;mean age±SD,41.63±14.53years).Collect and record clinical characteristics and laboratory indexes of every patients.Disease activity was evaluated by simplified Behcet's disease Current Activity Form(BDCAF)developed by The International Association for the study of Behcet's disease in 2006.Lymphocyte subsets and CD4+T cell subsets were detected by flow cytometry for all participants.According to the nearly 6 months whether application of glucocorticoids and thalidomide,hydroxychloroquine and other traditional antirheumatic drugs,eighty-nine patients were divided into antirheumatic drugs group(60cases)and Non-antirheumatic drugs control group(29 cases).Twenty-eight patients were treated with IL-2(50 WIU,subcutaneous injection,5 consecutive days).The lymphocyte subsets and CD4+T cell subsets were detected again at the end of the course of treatment.ALL the experimental data were analyzed by SPSS17.0 software.Results:1.Compared to healthy control,the absolute counts of CD8+T cells were increased(P=0.001),the percentage of CD8+T cells were also increased(P=0.000)in BD group,there were no difference between the absolute counts and the percentage of the two groups of T,B,CD4+T and T+B+NK.The percentage of NK cells were higher in healthy control,the absolute counts of NK cells was no difference for the two group.2.Compared to healthy control,the absolute counts and percentage of Th1 and Th17 cells were significantly increased in BD group,the absolute counts and percentage of Treg cells wrer decreased(P < 0.05),the ratio of Th1/Th2 was increased(P < 0.05),and the ratio of Th17/Treg were increased(P=0.000)in BD group.3.The absolute counts of T,B,NK,CD4+T,CD8+T,Th1,Th2,Th17 cells were not correlated with ESR and BDCAF scores,the absolute count of Treg cells were negative correlation with ESR and BDCAF score(r= 0.237,P < 0.05 VS r=-0.210,P < 0.05),Th1/Th2,Th17/Treg were not correlation with ESR and BDCAF score.4.The absolute counts of CD8+T cells,Th1,Th17 cells in antirheumatic drugs group were higher than healthy control,Th1/Th2 and Th17/Treg in antirheumatic drugs group were higher than healthy control(P<0.05 VS p=0.000),CD4+T/CD8+T in antirheumatic drugs group were lower than healthy control(P=0.000);CD4+T/CD8+T and Treg cells in non-antirheumatic drugs group were lower than healthy control,CD8+T cells,Th17 cells and Th17/Treg in non-antirheumatic drugs group were higher in healthy control.5.The absolute counts of B cells in Non-IL-2 group were lower in IL-2 group.CD8+T cells in Non-IL-2 group were higher than healthy control,but NK cells,CD4+T/CD8+T were lower in Non-IL-2 group than healthy control;The absolute counts of T cells and CD8+T cell were higher in IL-2 group than healthy control(P < 0.05 VS P=0.000),CD4+T/CD8+T were lower than healthy control(P < 0.005).6.The absolute counts of Treg cells in IL-2 group were higher than non-IL-2 group(p=0.000),Th17/Treg was lower in IL-2 group than non-IL-2 group(p=0.000),there were no difference in Th1,Th2 and Th17 cells between the two groups.Th1/Th2,Th17/Treg and Th17 cells were higher in IL-2 group than healthy control,the absolute counts of Treg cells were lower in non-IL-2 group than healthy control(p=0.000).The absolute counts of Th1,Th17,Treg cells and the rate of Th1/Th2 were higher in IL-2group than healthy control(p=0.000),no difference about Th17/Treg.Conclusion:1.The absolute counts of Th17 cells were increased while Treg cells were reduced,and Th17/Treg was imbalance in the peripheral circulation of patients with BD;2.Treg cells were negatively correlated with BD disease activity;3.Low dose IL-2 can selectively promote the proliferation of Treg cells and correct the imbalance of Th17/Treg. |
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