Sjogren's Syndrome In Patients With The Expression Of Th17 Cells And The Effect Runzaoling On Th17/Treg Cells

Objective1.By observing the expression of IL-17A in the Sjogren's syndrome(SS)patients' serum,and the correlation between their expression and TCM syndromes and laboratory items.2.To establish model of SS mice,using detoxification and dissipating blood stasis herbs to interpose,to test model of SS submandibular tissue and helper T cells(T helper cells,Th)17,regulatory T cells(regulatory T cells,Treg)in peripheral blood,to discuss the Th17/Treg immune balance role in the pathogenesis of SS and regulatory mechanism of detoxification and dissipating blood stasis herbs.Methods1.Choose 60 SS patients as experimental group,and choose 30 physical health persons as negative control group.Used the method of enzyme-linked immuno sorbent assay(ELISA)to detect the content of IL-17A in serum.2.Randomly 90 C57b1/6j mice were divided to six groups A,B,C,D,E,F,each group of 15,group A for blank,group B for model,group C for model and prednisone acetate,group D for model and Runzaoling High dose,group E for model and Runzaoling middle dose,group F for model and Runzaoling low dose.To establish model of SS mice.From the fourth week to infuse Runzaoing high?middle?low dose and prednisone acetate into mices gastric.After four weeks,mice were executed.2.2 Used the method of ELISA to detect the content of IL-17A in serum;used the method of reverse transcription-polymerase chain reaction(RT-PCR)to detect each groups submandibular tissue the expression of ROR? t?FoxP3;used the method of flow cytometry(FCM)to detect the expressing rate of Th17 and Treg cells.Results1.clinical research(1)ELISA result:Compared with the experimental group and the negative control group,the expression level of IL-17A,the experimental group were higher than the negative control group.(2)There were positive correlations of the expression levels of IL-17A and IgG,IgM in SS patients.(3)Positivity with ant-SSA antibody of SS patients the expression levels of IL-17A were higher than negativity with ant-SSA antibody of SS patients in serum(P<0.05);Positivity with ant-ANA antibody of SS patients the expression levels of IL-17A were higher than negativity with ant-ANA antibody of SS patients in serum(4)SS patients with Changjue gear and arthritis pain the expression levels of IL-17A in serum were higher than SS patients with non Changjue gear and arthritis pain.2.Animal experiments(1)Pathological section result:prednisone acetate and Runzaoling high dose can treat submandibular gland,thymus,spleen,lung and kidney-lesion.(2)Submandibular gland index determination result:prednisone acetate group,Runzaoing high dose group and Runzaoing middle dose group submandibular gland index were significantly increased than model group(P<0.05);there was no significant difference between with Runzaoing low dose group and model group(P>0.05).(3)ELISA result:model group mice and each treatment groups mice in the serum with the content of IL-17A were significantly increased than blank group(P<0.05);each treatment groups mice in serum with the content of IL-17A were significantly reduced than model group(P<<0.05).(4)RT-PCR result:Model group and each treatment groups ROR?t mRNA relative quantitative expression were significantly reduced than blank group(P<0.05),each treatment groups ROR ? t mRNA relative quantitative expression were significantly reduced than model group(P<0.05);Runzaoling high,middle,low dose groups ROR ? t mRNA relative quantitative expression were higher than prednisone acetate group(P<0.05).Each treatment groups FoxP3 mRNA relative quantitative expression were increased than blank group,model group FoxP3 mRNA relative quantitative expression was reduced than blank group with statistic significance(P<0.05);each treatment groups FoxP3 mRNA relative quantitative expression were increased than model group(P<0.05).(5)Flow cytometry instrument result:model group and Runzaoling middle,low dose groups CD4+T/CD4+CD25'T expression were higher than blank group,prednisone acetate group and Runzaoling high dose group CD4+T/CD4+CD25+T expression were lower than blank group(P<0.05).Conclusion:1.SS patients the expression of IL-17A in serum were significant 1y increased.There were positive correlations of the expression leve is of IL-17A and IgG,IgM,ant-SSA antibody,ant-ANA antibody.IL-17A co uld may be an important cause of SS.2.SS patients the expression of IL-17A in serum were significant ly increased.It could be caused Changjue gear and arthritis pain.3.Runzaoling can reduced IL-17A,and stimulated the proliferation of Treg cells,and inhibit the proliferation of Th17 cells in treatmen t and mechanism.Runzaoling can adjust the blance of Th17/Treg,and tr eat submandibular gland,thymus,spleen,lung and kidney lesion by autoi mmune dysfuncion.