Influence Of Edaravone On S100B Protein Level In Serum Of Neonatal Rats With Brain Damage Caused By Hyperbilirubinemia

Objective:To observe the serum S100 B protein level of neonatal rats with brain damage caused by hyperbilirubinemia and its concentration change after the intervention of edaravone.To probe into the effect of edaravone on brain injury caused by hyperbilirubinemia,and to provide certain experimental basis for edaravone in clinical prevention and treatment of bilirubin encephalopathy.Methods:A total of 96 7-day-old SD(Sprague-Dawley)rats were randomly divided into 3 groups:normal control group,hyperbilirubinemia group and edaravone intervention group,which namely group A,group B,group C,and each group had 32 rats.Each group was divided into 4 subgroups according to the different death times:24h group,48 h group,72 h group and 96 h group,and each subgroup had 8 rats.The rats in group B and group C were injected bilirubin of 100mg/kg by intraperitoneal injection for only 1 time.While the rats in group A were injected physiological saline with the same method.After modeling,the rats in group C were given intraperitoneal injection of edaravone immediately.And the dose of edaravone was 2 mg / kg /d,for 4 days.Finally collected the serum and brain tissue of rats of each group in different time points.HE(Hematoxylin and eosin)staining method was used to observe the pathological changes of brain tissue and measured the concentration of S100 B protein in the serum by Elisa method.Then recorded the experimental results,calculated the related data and carried out statistical analysis,and came to the conclusion.Results:(1)After modeling,the rats in group B and group C had the obvious neurobehavioral abnormalities,and the symptoms of rats in group C were significantly improved compared with those in group B at the different time points,but there was no significant abnormality of rats in group A.(2)24h after modeling,the brain tissue of rats in group B and group C had different levels edema,degeneration,inflammatory infiltration and so on,which became more serious with the extension of time.At the different time points,the changes of brain tissue of rats in group C were lighter than those in group B.While the brain tissue structure of rats in group A were basically normal.(3)The concentration of serum S100 B protein of rats in group B were higher than that in group A and group C,which were positively correlated with the time,there was statistically significant difference(P<0.05);the concentration of serum S100 B protein of rats in group C were lower than that in group B,and were higher than that in group A,there was statistically significant difference(P<0.05);the concentration of serum S100 B protein of rats in group A were lower,the difference was not statistically significant in the different death times within the group(P>0.05).Conclusion:(1)In the state of hyperbilirubinemia,the concentration of serum S100 B protein in rats are increased,which are positively correlated with the time;and the concentration of serum S100 B protein are significantly decreased after the intervention of edaravone.(2)In the state of hyperbilirubinemia,the brain tissue of rats have changes of edema,degeneration,inflammatory infiltration and so on after 24 h,and the degree of those changes are increased with the extension of time;after the intervention of edaravone,the changes of rats brain tissue can be reduced to a different extent at the different time points.(3)These results suggest that edaravone can relieve the damage of the central nervous system and have the effect on protecting neurons.