A Study On The Correlation Between Pre-thrombotic State And Recurrent Implantation Failure In Ivf-et

ObjectThe relationship between pre-thrombotic state and recurrent implantation failure was discussed by screening and analyzing the patients undergoing in vitro fertilization-embryo transfer and detecting of pre-thrombotic state.MethodsThe retrospective analysis was performed on the partial correlation indexes of the pre-thrombotic state(PTS)in the plasma samples of the patients with recurrent implantation failure(RIF)and the normal control group in the affiliated reproductive hospital of shandong university.The high risk factors of pre-thrombotic state were selected,including the activity of plasma protein C(PC)and protein S(PS),the activity of anti-thrombin III(AT-?),plasma homocysteine(HCY),lupus anticoagulant(LA),lupus anticoagulant test(LA)included lupus anticoagulant screening test(LA1),lupus anticoagulant identification test(LA2),standardized ratio of lupus anticoagulant(LA1/LA2).The activity of plasma protein C and protein S?activity of AT-??plasma HCY levels?LA1?LA2 and LA1/LA2 test results in patients and normal control group were collected,the main clinical data of each group and relevant laboratory test results were collected,then established an overall database for the collected data,classified and analyzed according to different groups.ResultsParticipants included 73 cases of recurrent implantation failure(RIF)as the case group(RIF group),in the same period,190 patients with recurrent spontaneous abortion(RSA)were treated as positive control group A(RSA group),at the same time,41 cases of normal fertility woman were served as negative control group B(normal control group).1.In the case group(RIF group),control group A(RSA group)and control group C(normal control group),there was a statistically significant difference between the high coagulation indicators of three groups:LA1?LA1/LA2?LA2?the activity of PS?plasma HCY levels(P<0.01).The difference of the activity of AT-? between three groups was statistically significant(P<0.05).There was no statistically significance of the activity of plasma protein C(P>0.05).2.The activity of AT-? of the case group(RIF group)and control group A(RSA group)was significantly lower than that in the normal control group.3.The activity of plasma protein S of the case group(RIF group)and control group A(RSA group)was significantly higher than that in the normal control group.4.Lupus anticoagulant screening test(LA1).lupus anticoagulant identification test(LA2)and standardized ratio of lupus anticoagulant(LA1/LA2)in the case group(RIF group)and control group A(RSA group)were significantly higher than that in the normal control group.The standardized ratio of lupus anticoagulant of the RIF group was 13.7%,similar to that of the RSA group(15.8%),which was much higher than the normal control group(0.0%).5.The HCY level of the control group A(RSA group)was significantly higher than that in the case group(RIF group)and there was no statistically significant difference between the normal control group and the case group(RIF group).Conclusion1.The detection of lupus anticoagulant in patients with RIF showed similar cases to the high coagulation state of patients with RSA,both were significantly higher than normal controls.Detection of high coagulation state in patients with RIF is beneficial to early treatment in patients with positive effect,which could help to improve the pregnancy outcome.2.The plasma HCY level of patients with RIF was similar to that of the normal control women,which was different from the high plasma HCY level of patients with RSA.3.Due to the lack of plasma AT-?,high coagulation state should be given sufficient attention to patients with recurrent pregnancy failure.