Association Of Serum IL-34 Concentration With Type 2 Diabetes And Obesity

Objective:To explore the association of serum IL-34 concentration with type 2diabetes and obesity.Methods:99 were chosen into two groups according to 1998 year WHO obesity guide,the nonobesity was 32 and the obesity was 67,?the nonobesity arranged from 19.1kg/m² to 24.9 kg/m²,the obesity was equal or larger than 25 kg/m²?.The first group was divided into two subgroups,subgroup 1?n=17?was without T2DM,subgroup 2?n=15?was with T2DM.The second group was also divided into two subgroups,subgroup 3?n=33?was without T2DM,subgroup 4?n=34?was with T2DM.Serum IL-34concentration,hsCRP,fast insulin?FINS?,fast plasma glucose?FPG?,total cholesterol?TC?,triglyceride?TG?,low density lipoprotein cholesterol?LDL-C?,high density lipoprotein cholesterol?HDL-C?,HbA₁c were measured,to determine systolic blood pressure,diastolic blood pressure,waistline circumference?WC?,hipline circumference?HC?,age,sex,calculate HOMA-IR,BMI.Then serum IL-34 concentration was measured using commercial enzyme-linked immunosorbent assay?ELISA?kits according to the manufacturer's instructions.Results:Data analysis of 99:Serum IL-34 concentration of the subgroup 3 was higher than subgroup 1,2 and 4.The subgroup 4 was also higher than the subgroup 1 and subgroup 2.Subgroup 1 and 2 had no statistical significance.The serum IL-34concentration of subgroup 1,2,3,4 was respectively?167.1±39.38?vs?170.71±35.68?vs?244.48±42.2?vs?211.44±23.83?ng/L.Pearson correlation analysis showed serum IL-34concentration was positively correlated with hsCRP,fast insulin,HOMA-IR,WC,HC,BMI?r=0.672,0.442,0.566,0.678,0.476,0.651,P?27?0.01?.Multiple stepwise regression analysis showed waistline circumference,BMI was independent influencing factors for serum IL-34 concentration??=0.434,P?27?0.01;?=0.321,P?27?0.01?.After one month of laparoscopic Roux-en-Y gastric bypass?RYGB?surgery of 8?5 women and 3 men?from the group of obesity,hsCRP,BMI,serum IL-34 concentration,waistline was lower,only HOMA-IR had statistical significance.Conclusion:?1?The serum IL-34 concentration is significantly higher during obesity,in obesity group it almost has no relationship with T2DM,so the association between IL-34 and T2DM is unclear.?2?There existed a positive correlation between IL-34 and hsCRP,FINS,HOMA-IR,WC,HC,BMI,and WC and BMI are the independent factors of serum IL-34 level,it may have association with insulin resistance and inflammation,which reveals we can speculate visceral adipose tissue by testing serum IL-34 in order to formulate a corresponding weight loss plan.