Upregulation Of MicroRNA-383 Inhibits Proliferation,Migration And Invasion Of Colon Cancer Cells

Objective:Increasing number of evidence show that miRNA,a type of non-encoding small RNA,can regulate tumor cell migration,invasion,and metastasis,and play a significant role in the occurrence and development of tumor.The current study mainly investigates the effect of miR-383 on proliferation,migration,and invasion of colon cancer cell lines HT-29 and LoVo.Methods:The expression of miR-383 in colon cancer and adjacent non-tumor tissues was examined by real-time PCR.miR-383 expression was up-regulated by rno-miR-383 mimic.Cell proliferation was detected by MTT assay and clonal methods,cell migration and invasion potential were examined by Transwell Chamber and Matrigel invasion chamber assays.APRIL,MCL-1,and COX-2 proteins expression were detected by Western blotting.Results:The expression of miR-383 was decreased in colon cancer tissues compared to the adjacent non-tumor tissues(P<0.05).Up-regulated miR-383 suppressed the proliferation,and inhibited the cell migration and invasion in HT-29 and LoVo colon cancer cell lines.Overexpression of miR-383 in HT-29 and LoVo cells resulted in suppressing the expression of APRIL protein.Conclusion:miR-383 was down-regulated in colon cancer.Up-regulated miR-383 inhibited proliferation,migration,and invasion of colon cancer;inhibition may be achieved through the regulation of target gene APRIL.