| ObjectiveTo analyze the changes in thromboelastography(TEG)and platelet(PLT)parameters in NSCLC and explores the effect of the tumor on the number and functions of circulating platelets.Then,determining the changes in CD62 P expression,a platelet activation marker,to understand its role in lung cancer.Methods1.The object of studyWe included 70 LC patients(LC group)who were hospitalized at the Jinan Military Region General Hospital from September 2016 to December 2017 and 70 healthy subjects(control group)during the same period.All cases were required not to use blood coagulation drugs 2 weeks before blood collection.2.MethodsFasting venous blood samples were taken from upper arm in the morning.TEG was performed by thromboelastometry(TEG-500,USA).PLT were counted by an automatic analyzer.CD61 and CD62 P were detected by flow cytometry.3.Statistical methodUsing SPSS 17.0 statistical software,measurement data were analyzed by independent sample t-test.One-way ANOVA was used to compare the data between different groups.The ?2 test was used to process the count data.P<0.05 was considered as statistically significant.Results1.The values of MA in TEG were higher in LC group than those in control group;the count of PLT was significantly increased,PDW and MPV were also obviously reduced(P<0.05).2.According to the clinical TNM stage,the lung cancer was divided into 20 cases of A group,that is stage and ? ?,and 20 cases of B group,that is stage ? and ?.There were significant differences in PLT,PDW and MPV between the A group and the control group(P<0.05).The differences of MA,PLT,PDW and MPV between the B group and the control group were statistically obvious(P<0.05).Comparison of A and B group showed that only the MA value increased significantly(P <0.05).3.According to the patients with or without lymph node metastasis,LC group was divided into 17 cases without lymph node metastasis(N0)group and 23 cases with lymph node metastasis(N1)group.The difference of MA,PLT,PDW and MPV between N1 group and control group were statistically significant(P<0.05).There were no obvious differences in K,MA and CI between N0 group and control group(P>0.05).And the other indexes of PLT,PDW and MPV were all statistically different(P<0.05).Compared with N0 group,MA value obviously increased in N1 group(P<0.05)4.The count of PLT in LC group was significantly higher than that in control group,(250.60±57.09)×109/L vs.(192.35±43.37)×109/L(P<0.01).There was no significant difference in PLT count between lung squamous cell carcinoma and lung adenocarcinoma(P> 0.05).The positive rate of CD62 P in LC group was significantly higher than that in control group,(50.42±15.42)% vs.(27.31±18.16)%(P<0.01).The positive rate of CD62 P was(50.11±13.48)% in lung squamous cell carcinoma,and(50.73±17.63)% in lung adenocarcinoma.There was no significant difference between the two groups(P>0.05)),but both were obviously higher than that of the control group(P<0.05).5.Except that stage ?(P>0.05),PLT counts of stage ?,?and ?were significantly higher than that of control group(P<0.05).Also,PLT count of stage ? was obviously higher than that of stage ?(P<0.05).Compared with the control group,the positive rates of CD62 P in stages ?,?,?and ?were significantly increased(P<0.05).There was significant difference between stage ? and stage ?(P<0.05).ConclusionsNSCLC plays an important factor in the increased number of PLT and enhancement of their function.In particular,PLT function was more significantly changed in patients with late tumor stage or lymph node metastasis.PLT function is highly expressed in lung squamous cell carcinoma and lung adenocarcinoma.Although,PLT count in cases of stage ? did not change significantly,its function has been significantly activated.With the increase of staging,the number of PLT increased and the rate of functional activation rised.The enhancement of PLT adhesion and aggregation function is more common in NSCLC,which is conducive to the metastasis and invasion of lung cancer cells. |
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