Expression And Clinical Significance Of Twist,C-myc In Colorectal Cancer

ObjectiveTo study the expression and clinical pathological significance of epithelial-mesenchymal transition factor Twist(basic helix-loop-helix transcription factor)and proto-oncogene C-myc(nuclear regulatory protein short-circuit activating factor)in human colorectal cancer.MethodsIn this study,Immunohistochemistry SABC method and Western blot method of 80 cases of colorectal cancer tissue and its adjacent normal mucosa(more than 5cm away from the cancer tissue)Twist,C-myc protein localization and semi-quantitative detection and analysis the relationship between them and their relationship with clinical biological behavior.Results(1)The positive rates of Twist and C-myc in colorectal cancer tissues were significantly higher than those in normal colorectal mucosa(P <0.05).(2)The expression of Twist protein was correlated with tumor differentiation,lymph node metastasis,histopathological grade and TNM stage(P <0.05),but not with age,sex and tumor size(P> 0.05).(3)The expression of C-myc was correlated with lymph node metastasis and TNM stage(P <0.05),but not with tumor differentiation,age,gender and tumor size(P> 0.05).(4)Overexpression of C-myc protein was found in the overexpression ofTwist protein,and the expression of C-myc was positively correlated(r = 0.454,P <0.05).ConclusionsThe expression of Twist and C-myc in colorectal cancer tissues was highly expressed,which had a certain relationship with clinicopathological features,and there was a positive correlation between them,indicating that both of them may play an important role in the carcinogenesis and invasion and metastasis of colorectal cancer to promote the role,and synergistic to promote the occurrence and development of tumors.