| Objective:(1)To analyze the main factors affecting the quality of life of patients through investigating the questionnaire of the demographics,pain,and quality of life in brucellosis patients;(2)To investigate the relationship between pain symptoms affecting patients’ quality of life and plasma Th1/Th2 cytokines through detecting Th1/Th2 cytokine expression levels by flow cytometry.Methods:(1)Using Self-made demographic data of brucellosis patients,Life Quality Scale(SF-36),and Facial Expression Pain Assessment Scale for 524 patients with brucellosis who were admitted to the hospital according to the inclusion and exclusion criteria were surveyed in Xinjiang Uygur Autonomous Region Infectious Disease Hospital from January 2017 to December 2017,to evaluate the demographic data,pain and quality of life and collect blood samples;(2)A total of 128 patients with brucellosis accompanied by varying degrees of pain and painless were randomly selected from blood samples of 524 patients,thirty-two healthy people who met the inclusion and exclusion criteria were selected from the staff of the Xinjiang Uygur Autonomous Region Infectious Disease Hospital.A total of 160 subjects were divided into five groups(mild pain group,moderate group,severe pain group,painless group,and a healthy control group);(3)A total of 160 subjects’ blood sample was tested for Th1/Th2 cytokines by flow cytometry.Results:(1)Investigating the baseline data of brucellosis patients found that 85.5% of patients experienced pain symptoms;the quality of life survey found that compared with other factors,whether the occurrence of pain is the main influencing factors in the physiological function and total score of the quality of life(all P<0.01).(2)The result of flow cytometry showed that the expression levels of IFN-γ,TNF-α,IL-6(all P<0.01)and IL-2(P<0.05)were different before and after treatment in 128 patients;There was a statistically significant decrease in the expression levels of IFN-γ,TNF-α,and IL-6 cytokines before treatment;The IL-2 level was significantly higher than that before treatment;IL-10 and IL-4 has no significant change compared with before treatment.(3)The results of flow cytometry showed that compared with the healthy control group,the expression level of IFN-γ,TNF-α,IL-10,IL-6,IL-4(all P<0.01)and IL-2(all P<0.01)in severe pain patients before treatment was statistically significant;the expression level of IFN-γ,TNF-α,IL-6,IL-4 and IL-2 in moderate pain patients before treatment was statistically significant(all P<0.01);the expression level of IFN-γ,TNF-α,IL-10,IL-6 and IL-4 in mild pain patients before treatment was statistically significant(all P<0.01);the expression level of IFN-γ,IL-6,IL-4 and IL-2(all P<0.01)in painless patients before treatment was statistically significant;the expression level of IFN-γ,IL-6,IL-4,IL-2(all P<0.01)in severe pain patients after treatment was statistically significant;the expression level of IFN-γ,TNF-α,IL-6,IL-4 and IL-2(all P<0.01)in moderate pain patients after treatment was statistically significant;the expression level of IFN-γ,TNF-α,IL-10,IL-6,IL-4 and IL-2(all P<0.01)in mild pain patients after treatment was statistically significant;the expression level of IFN-γ,TNF-α,IL-6 and IL-4(all P<0.01)in painless patients after treatment was statistically significant.Conclusion:(1)Pain is the main influencing factor of the quality of life of patients with brucellosis.(2)The pain of patients with brucellosis is closely related to the expression levels of IFN-γ,TNF-α,IL-6 and IL-2.(3)After a month treatment,the expression levels of IFN-γ,TNF-α and IL-6 cytokines were significantly lower than those before treatment,and the expression levels of IL-2 were significantly higher than those before treatment.There was no significant change in IL-10 and IL-4 between prior treatment and posttreatment.(4)Compared with the healthy control group,the expression of Th1/Th2 cytokines was also different before and after treatment in patients with different degrees of pain. |
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