Eeffects Of Overexpressed CacyBP/SIP On Biological Function Of Eutopuic Ovarian Endometrial Stromal Cell

Objectives:To investigate the influence of over-expression CacyBP/SIP on the biological function of eutopuic ovarian endometrial stromal cell.Methods:1.Extracorporeal extract eutopic endometrial stromal cells from the patients with ovarian endometriosis,and we used recombinant CacyBP/SIP lentivirus to transfect these cells then we detected the result of virus transfection.2.The experimental set three groups : Lv-hCacyBP/SIP group which infected with recombinant lentivirus CacyBP/SIP(Lv-hCacyBP/SIP),Lv-control group which infected with recombinant lentivirus vector and a blank group(untreated group).3.The cell proliferation was detected by CCK-8 method.4.Transwenll was used to detect cell migration and invasion ability.5.The cell apoptosis was detected by flow cytometry.Results:1.In vitro isolation and culture the Ovarian endometriosis symptoms,eutopic endometrial stromal cells.We have successfully obtained the eutopic endometrial stromal cells.2.The recombinant lentivirus successfully was constructed,detecting the expression of CacyBP/SIP in all experimental cells after transfection.The expression of CacyBP/SIP in Lv-hCacyBP/SIP group(0.676±0.031),Lv-control group(0.251±0.001),untreated group(0.250±0.007),the differences are statistically significant between the comparison of Lv-hCacyBP/SIP group and Lv-control group(t=21.231,P<0.001)and there was no statistically significant between untreated group and Lv-control group(t=0.109,P=0.921).3.The results of cell proliferation test by CCK-8 method showed that after the culture of 24 h,the A value of Lv-hCacyBP/SIP group(0.247±0.007)compared withLv-control group's(0.260±0.004)has no significant difference(t=2.641,P=0.057).After the culture of 48 h,the A value of Lv-CacyBP/SIP group(0.343±0.002)was significantly lower than that of Lv-control group's(0.376±0.005)(t=9.535,P=0.001).4.The Cell Migration ability was detected by Transwell.The migration number of Lv-hCacyBP/SIP group cells were(134±21.5),which were significantly lower than that of Lv-control group cells(224±12.0),the difference was statistically significant(t=6.338,P=0.03).However,there was no significant difference in the number of cells between Lv-control group(224±12.0)and untreated group(226±15.2)(t=0.207,P=0.846).5.The Cell invasion ability was detected by Transwell.The migration number of Lv-hCacyBP/SIP group cells were(92.33±6.8),which were significantly lower than that of Lv-control group cells(128.67±4.7),the difference was statistically significant(t=5.713,P=0.029).The number of cells between Lv-control group(128.67±4.7)and untreated group(125.33±10.1)was no significant difference(t=0.459,P =0.691).6.The apoptosis was detected by flow cytometry method.The cells apoptosis rate of Lv-hCacyBP/SIP group was(8.066±2.85)% and Lv-control group was(8.865±0.03)%,there was no statistically significant between Lv-hCacyBP/SIP group and Lv-control group(t=0.481,P=0.677).Lv-control group(8.865±0.03)%and untreated group(8.433±1.48)%,there was no statistically significant between Lv-control group and untreated group(t=0.490,P =0.650).Conclusion:Overexpression of CacyBP/SIP could inhibit the proliferation,migration and invasion of OEMs eutopic stromal cells,but has no effect on apoptosis.