The Study Of Protective Effect And Mechanism Of Sulforaphane On Human Umbilical Vein Endothelial Cell Injury Induced By Hydrogen Peroxide

Objective:To investigate the protective effect of sulforaphane?SULFORAPHANE,SFN?on oxidative damage of human umbilical vein endothelial cells?HUVEC?induced by hydrogen peroxide?H₂O₂?,and to further study the mechanism of sulforaphane in the protection of endothelial cell injury.Methods:The human umbilical vein endothelial cell line provided by Shanghai cell bank of Chinese Academy of Sciences was resuscitated,and the cells were cultured at 37 5%CO₂and over 80%degree of fusion.Experimental groups:?1?Blank control group?Control group?:The cells were cultured for 2.5 hours.?2?Hydrogen peroxide group?group H₂O₂?:To give separately 100.200.400.600.800?M cells were cultured for 2 hours,and 400?M was selected as the best intervention concentration.?If the following groups are not specified,the default H₂O₂ concentration is 400?M?;?3?Sulforaphane+hydrogen peroxide group?group SFN+H₂O₂?:Different concentration of SFN2.5.5.10?M)after incubating for 30 min,2h was continued to be cultured with 400?M H₂O₂.To collect the cells after intervention,the expression of intracellular transcription factor E2?NRF2?mRNA and heme oxygenase-1?HO-1?mRNA was determined by using MTT assay to compare cell activity,flow cytometry assay of intracellular ROS levels,and reverse transcriptase polymerase chain reaction?RT-PCR?.Results:Compared with the control group,the cell absorbance value?a value?of H₂O₂ group was significantly reduced?p<0.05?,and the value of a was decreased by the concentration dependence,and the absorbance value of SFN+H₂O₂ group was higher than that of H₂O₂Group?p<0.05?.The level of ROS in H₂O₂ group was higher than that in control group?p<0.05?,and the ROS level in SFN+H₂O₂ group was lower than that in H₂O₂ Group?p<0.05?.The expression level of Nrf2 mRNA and HO-1 mRNA in H₂O₂ group was lower than that in control group?p<0.05?,and HO-1 m RNA and Nrf2 mRNA expression in SFN+H₂O₂ group were higher than that in H₂O₂ Group?p<0.05?.Conclusion:Sulforaphane has protective effect on oxidative damage of HUVEC caused by hydro-gen peroxide,and its mechanism may be related to the expression of sulforaphane in trans-cription level activation Nrf2/HO-1.