The Effects And Mechanisms Of GSA On Expression Of MCP-1 In Cultured Human Umbilical Vein Endothelial Cells

Objective:To investigate the effects and mechanisms of glycated serum albumin(GSA)on expression of Monocyte chemoattratant protein-1(MCP-1)in endothelial cells.Methods:In present study,the Human Umbilical Vein Endothelial Cells(HUVEC) cultured with or without N-acetylcysteine(NAC)were exposed to GSA,and then the expression of MCP-1 were determined by Immunocytochemistry and ELISA, SOD and MDA were detected by the technique of XOD and TBA respectively.Result:(1)GSA can stimulate the production and secretion of MCP-1.The expression of the MCP-1 increases gradually when HUVECs were exposed to GSA with a time and dose-dependent manner,(2)GSA can cause the decrease of SOD and the increase of MDA in HUVECs.When HUVECs were exposed to different dose of GSA for 12 hours,the activity of SOD in HUVECs decreased obviously while the content of MDA increased,with a dose-dependent manner.(3) NAC can restrain the effect of GSA on the expression of MCP-1 in HUVECs. After HUVECs were pretreated by NAC,the expression of MCP-1 in HUVECs stimulated by GSA decrease obviously.(4)NAC can restrain the effect of GSA on the activity of SOD and the content of MDA in HUVECs.After HUVECs were pretreated by NAC,the effects of GSA on SOD activity and MDA content in HUVECs were blocked.Conclusion:(1)GSA can stimulate the production and secretion of MCP-1 of Human Endothelial Cells.(2)GSA can induce Human Endothelial Cells oxidative stress.(3)The biological effects of GSA on stimulating MCP-1 expression may be mediated by oxidative stress.(4)Anti-oxidants are expected to be effective approach of prevention and treatment for diabetic vascular complications.