Preliminary Study On Extracellular Signal-Regulated Kinase 1/2 Activation And Related Protein Expression During The Development Of Gastric Cancer

Objective To observe the infection of Helicobacter pylori(Hp)in chronic superficial gastritis,chronic atrophic gastritis,intestinal metaplasia,intraepithelial neoplasia,early gastric cancer and advanced gastric cancer,and the activation of phosphorylated extracellular signal-regulated kinase 1/2 Related proteins c-myc,c-Fos,ATF4 expression characteristics of gastric cancer during the development of extracellular signal-regulated kinase and its mechanism.Methods Immunohistochemical staining was used to detect chronic superficial gastritis(20 cases),chronic atrophic gastritis(20 cases),intestinal metaplasia(19 cases),low grade intraepithelial neoplasia(20 cases),high grade intraepithelial neoplasia The expression of P-ERK1/2,ERK1/2,c-myc,c-Fos and ATF4 in early gastric cancer(31 cases)and advanced gastric cancer(73 cases)Semiquantitative evaluation of the expression of P-ERK1/2,ERK1/2,c-myc,c-Fos,ATF4 in each group.Results In each group of immunohistochemistry result show: The expression of HP in high magnification in gastric pits or glandular cavity visible brown short rod,comma shaped or spherical.In chronic superficial gastritis(55%),intestinal metaplasia(47.4%),high grade intraepithelial neoplasia groups(41.7%),early gastric cancer(16.1%)and advanced gastric carcinoma(8.2%),the infection rate of Hp was lower than the chronic atrophic gastritis(94.7%)(P<0.05).The expression of P-ERK1/2 and ERK1/2 was positive in cytoplasm or membrane,and the expre-ssion of P-ERK1/2 in advanced gastric cancer was higher than that in mild chronic gastritis(15.0%)and intestinal metaplasia(15.8%(P<0.05).The expression of P-ERK1/2 in early gastric cancer was higher than that in chronic gastritis and chronic atrophic gastritis(35.0%)And intestinal metaplasia(P<0.05).c-myc was located in the nucleus.The expression of c-myc in advanced gastric cancer(34.2%)was lower than that in low-grade intraepithelial neoplasia(60.0)(P<0.05).c-Fos positive cells located in the cytoplasm and membrane,advanced gastric cancer(83.6%)c-Fos expression rate was higher than low-grade intraepithelial neoplasia(35.0%),chronic superficial gastritis(50.0%),chronic atrophic gastritis(26.3%)(P<0.05).The expression of c-Fos in early gastric cancer(67.7%)and high-grade intraepithelial neoplasia(75.0%)was higher than that in low-grade intraepithelial neoplasia and atrophic gastritis(P<0.05).The positive rates of P-ERK1/2,ERK1/2,ATF-4,c-myc and c-Fos in gastric cancer were statistically significant compared with non-cancer group(P<0.05).Compared with Hp infection group and Hp noninfection group,the expression rates of P-ERK1/2,ERK1/2,c-myc and c-Fos in Hp-positive group were higher than those in Hp-negative group,Hp-positive P-ERK1/2 was higher in the low-grade intraepithelial neoplasia than in the Hp negative group,and the AFP4 in the Hp-positive group was higher in the advanced gastric cancer group than in the Hp negative group(P<0.05).Conclusion Hp infection is related to the expression of ERK1/2,P-ERK1/2,c-myc,c-Fos and ATF-4 during the development of gastric cancer.