| The drug abuse and smuggling of methamphetamine(MA) have increased year by year in the world though it has been strictly controlled, which can bring significan t impacts on human health and social security. Therefore, the detection of trace MA i s given a pressing concern for combating criminals and maintaining social stability. In the practice,the colloidal gold immune chromatography is used to determine the fact s of drug use, and meteorological chromatography- mass spectrometry is used to dete ction the content of methamphetamine. Recently, the quantitative analysis of MA is ca rried out with fluorescence and chemiluminescence, which have been reported because of their high sensitivity, fast response and low-cost. However, several defects are hardl y avoidable such as complex for sample pre-preparation, low selectivity, high testing cost s and expensive apparatuses. Immunosensor combines both advantages of immune respon se and sensors, effectively improve the specificity and sensitivity of detection. One of the most frequently used methods is enzyme-linked immune sensor, which exhibits so me disadvantage factor such as the loss of mediators, short lifespan. To avoid the abo ve shortcomings, Prussian blue(PB) is applied to catalysis reduction of H2O2 to amplif y the amperometric signal of the immunosensor and platinum nanoparticles are used a s electron transfer mediator. The current response could be effectively enhanced by co deposition of PB and platinum nanoparticles. Because PB is unstable in aqueous solu tion, 2d-net MPS hydrolyzed and condensed was dropped on the surface of the PB-m odified electrode, forming a stable film that protected the PB film against leaking. Th e obtained immunosensor has more obvious advantages such as excellent selectivity, g ood specificity, high sensitivity and low cost. This method offers an economical, simple and convenient way to obtain high-quality detectors for the detection of MA. When s ensors to realize miniaturizationm, it can be made into a portable test equipment, whic h could achieve instant and fast detection for methamphetamine. Moreover, the sensor ar ray can be made for high-throughput detection of methamphetamine. So the immunose nsor has enormous development potentials in the field of methamphetamine determination..In this paper, through the analysis of the reference material and the literature anal ysis, inductive analysis, experimental method, comparative analysis, we summed up and summarized the traditional and emerging method, finding that there are some proble ms and defects in the existing detection methods, and trying to explore the new meth ods and technology to solve the problem. We found that the immunosensor has some advantages compared with common methods, such as excellent selectivity and high sen sitivity. Finally, we obtained anti-MA/nano-Au/2d network of MPS/nano PB&nanoPt-mo dified electrodes. Finally, experimental results show that the immunosensor in the dete ction of MA has feasibility and superiority. This paper is divided into five parts to ex plain.The first part, the introduction, mainly introduces latent status of detection of MA,the advantages and disadvantages of existing methods, and shows research method of thesis writing, theoretical and practical significance of research and development tren d.The second part introduces the composition of MA and its form in sample.The third part, introduces the traditional methods, the National Standard Methods a nd the emerging methods of detection of MA, and shows their defects.The fourth part, introduces principle of label-free amperometric immunosensor for the detection of MA and shows the preparation of it. PB exhibited excellent electrocat alytical properties toward the reduction of H2O2 and nano-Pt was used as electron tran sfer mediator to amplified the amperometric signal. The amperometric immunosensor, c onstructed by antibody/nano-Au/2d network of MPS on the nano-PB&nao-Pt –modified electrodes, was developed for the detection of MA, which enhanced the sensitivity, s pecificity, stability of the immunosensor. Under optimal immunoassay conditions, the relationship between the change in amperometric response and the concentration of M A was linear in the concentration range of 1.0×10-8-5.0×10-6mol /L with a correlation coefficient of 0.9943(n=4), linear regression equation for I=-0.4894X+2.7963.The fifth part, summarized the application of amperometric nanopaticles showing s uperiority but still has some shortcomings in detection of MA. And hoping that throu gh further development and mature technology, we can realize miniature and high-thro ughput sensors, and achieve fast-field analysis. |
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