Effects Of Aerobic Exercise On TNF-α、IL-6 And P38MAPK Signal Pathway In The Skeletal Muscle Of Type Ⅱ Diabetic Rats

Objective: TypeⅡdiabetic model were acquired with streptozocin injection to high-fat diet feeding rats,to observe the movement interference on inflammatory cytokines in diabetic rat skeletal muscle tissue TNF-a,IL-6 and the expression level of p38 MAPK and the phosphorylated p38 MAPK protein.To investigate the therapeutic effect of exercise on type II diabetes and to investigate whether exercise can be carried through the anti-inflammatory mechanism to reduce insulin resistance in skeletal muscle to improve the development of type 2 diabetes mellitus.For providing scientific basis and reference for the treatment of type II diabetes mellitus.Methods: 50 male SD rats which are 6 weeks old,randomly selected 10 rats as normal control group(NC),the rest 40 rats feeding high-fat diet,by intraperitoneal injection of 35 mg/kg chain urea with STZ type Ⅱ diabetic rats model was established.The success of the model rats are swimming training,selecting 20 were randomly divided into diabetic control group(DC),diabetes(DE)aerobic exercise group.Diabetes aerobic exercise group of swimming for 6 weeks.The experimental group rats fasting blood glucose,serum insulin and skeletal muscle inflammation factor in TNF-a and IL-6 level change and P38 lightning protein phosphorylation and protein expression level of change.Results:(1)DC group rats FBG,fins,insulin resistance index and NC group comparison showed very significant of elevated(P < 0.01);de rats FBG,fins and insulin resistance index and DC group comparison showed a decreasing trend,and has significant difference(P < 0.01).(2)the concentration of skeletal muscle in DC rats of TNF-were compared with the NC group significantly increased,with statistical significance(P<0.01).The concentration of IL-6 in skeletal muscle of rats in DC group was significantly higher than that of NC group(P<0.05).The skeletal muscle of DE rats in TNF-a levels compared with DC group were significantly decreased(P<0.05),while the concentration of IL-6 increased after exercise,and have statistical significance(P<0.05).(3)there was no significant difference between the expression level of each rat skeletal muscle p38 protein(P > 0.05).The level of phosphorylated p38 in skeletal muscle of NC group was higher than that in NC group,and the difference was statistically significant(P<0.05).The level of phosphorylated p38 in skeletal muscle of DE group was significantly higher than that in group DC(P<0.01).(4)the expression of GLUT4 protein in skeletal muscle of NC group was higher than that in DC group,and the difference was statistically significant(P<0.05).The expression of GLUT4 protein in skeletal muscle of DE group was significantly higher than that in group DC(P<0.05).Conclusion:6 weeks aerobic swimming exercise can effectively reduce type II diabetic rats blood glucose,improve glucose metabolism in type II diabetic rats,reduce the level of insulin resistance.Exercise has anti-inflammatory effect,aerobic exercise increased the type II diabetic rat skeletal muscle tissue of anti-inflammatory cytokine IL-6 levels,reduce the inflammatory factor TNF-levels,and through activation of p38 MAPK phosphorylation level increases the expression of GLUT4 protein content,so as to improve the skeletal muscle glucose uptake and transport,to improve glucose metabolism,improve IR.