The Effect Of Carbohydrate Supplementation On Reproductive Hormone And Relevant Energy Metabolism Hormone In Female Athletes With EAMD

Objective: This study was based on the use of energy hypothesis,adopting the way of carbohydrate supplementation to intervene Exercise-Associated Menstrual Disorders?EAMD?without disturbing their training and daily life.Discussing the effect of carbohydrate supplementation on energy status and the changes in body energy expenditure on the reproductive hormones,related stress hormones and energy metabolism hormones,and try to explore its correlation and influencing mechanism to observe the effect of carbohydrate supplement on EAMD.Method: Fourteen participants were randomly assigned into experimental carbohydrate group and control group with 7 for each.Experimental group was given carbohydrate intervention for 3 consecutive months.Body composition and energy expenditure were measured in both pre-and post-study for all participants.Venous blood was collected during follicular phase and luteal phase in menstrual cycle prior to the intervention and the third menstrual cycle during the experiment respectively,aimed at measuring the release of Gn RH,FSH,LH,E₂,P,T,Ghrelin,Leptin,C,TC,TG,HDL-C and LDL-C.Discussing how the effect of carbohydrate supplementation on EAMD by comparing the energy balance of the subjects pre-and post-study and the changes of the relevant hormone.Result: 1.All the participants were in negative balance before the study?experimental group:-468.9±367.1 kcal;control group:-445.6±304.8 kcal?.The experimental group achieved positive energy balance after intervention?132.5±107.8 kcal?.The significant difference was found between the two groups,which was also found within experimental group statistically.?p< 0.05?.However,no significant difference was found within control group?p>0.05?as negative energy balance was identified in its pre-and post-study.2.The release of E₂ and P elevated in follicular phase and luteal phase respectively after the carbohydrate intervention?E₂:52.4±6.5⁸8.5±41.1;P:6.7±7.2⁷.7±5.1?,different from control group significantly?p<0.05?;Moreover,there is no significant difference was observed in the release of Gn RH?FSH?LH?T?Ghrelin?Leptin?C?TC?TG?HDL-C?LDL-C after intervention.3.The correlation analysis showed that GnRH had positive correlation with TC and LDL-C,and LH,T had positive correlation with TC and HDL-C? Moreover,FSH and P were positively correlated with HDL-C.Conclusion: 1 Carbohydrate supplementation could help EAMD participants with negative energy balance change into positive energy balance,indicating that there was a good effect on the lack of energy in the body,which could reverse the negative balance of energy.2 The release of reproductive hormone E₂ and P elevated in follicular phase and luteal phase respectively after the carbohydrate intervention,which can help people with EAMD returned to normal menstrual cycle.3 There is a certain correlation between reproductive hormone and cholesterol in this study: Gn RH and TC,LDL-C had a moderate positive correlation.LH,T and TC,HDL-C had a positive correlation.FSH and P had a positive correlation with HDL-C.These results indicated the relationship between cholesterol and the reproductive hormone secreted by HPO tissue at all levels,and it had effect on normal menstrual cycle.