| Objective:As the chronic negative energy balance is the primary factor contributing toathletic menstrual cycle irregularities (AMI). The purpose of this study was todetermine if carbohydrate supplementation improves the rats with estrogen cycleinhibition by the level of serum hormone concentrations and the changes of Lipidmetabolism. It’s the important theoretical basis for athletic menstrual cycleirregularities athletes on dietary recommendations.Method:The experiment was divided into two parts, through regular observation vaginalexfoliated cells to determine the rat estrous cycle status. The first part of36twomonths old SD rats were randomly divided into four groups○1The amenorrhoeamodel group, which was normal diet and6weeks Incremental Load Training, M;○2Continue for six weeks Incremental Load training, normal diet and supplemented withglucose at the same time,6P;○3Continue for six weeks Incremental Load training,normal diet and supplemented with oligosaccharides at the same time,6D;○4Congroup, Normal diet and no exercise, the negative control group.The second part of54two months old SD rats were randomly divided into threegroups. The first group was blank group (Con group, Normal diet and no exercise, thenegative control group)(With the first part); The second group was the amenorrhoeamodel group (M group, Normal diet and6weeks Incremental Load Training, positivecontrol group)(the same as M of the first part); The third group was takenintervention after6weeks Incremental Load Training, which include○1Quiet rest forthree weeks,3Q;○2Continue for three weeks training, the intensely was the same asthe sixth week’s,3U;○3Continue for three weeks training, the intensely was the sameas the sixth week’s, supplemented with glucose at the same time,3P;○4Continue forthree weeks training, the intensely was the same as the sixth week’s, supplementedwith oligosaccharides at the same time,3D.With the reference to Bedford animal exercise load standards,3D group,3Pgroup,3U group had done exercises for nine weeks,3Q group recovered quietly aftersix weeks; M group,6D group and6P had done exercises for nine weeks,3weeksbehind other groups. One more exercise was added to the rats’ last training. Then allrats were put to death after a36hours break, in which the last eight hours without diet;firstly took5ml blood from inferior vena cava, after separating serum, theprogesterone (P), leptin were tested; TC、TG、LDL、HDL、GLU were tested; andtake the white adipose tissue from the around of kidneys, abdominal wall andreproductive organ.Results:1. Group3D and Group3P compared with Group C:Visceral fat weight, TG and LDL-c of3D and3P group were significant higherthan C group; The lee’s index was significant lower than C group, but3P group wasnot significant difference with C group; TC, E2and P were not significant differencecompare with C group; The leptin level of3D group was significant lower than C group, but3P group was not significant difference with C group; The HDL-cconcentration of3D and3P group were higher than C group, but no significant; Bloodglucose concentration was significant higher compare3P group with C group, but nosignificant difference in3D group and C group.2. Group3D and Group3P compared with Group M:Body weight, visceral fat weight, P, leptin level, TC, TG, HDL-c, LDL-c andblood glucose concentration of3D and3P group were significant higher than M group;The E2of3D group were significant higher than M group, but3P group was notsignificant difference with M group; lee’s index was not significant difference.3. Group3D and Group3P compared with Group3Q:Body weight, visceral fat weight, TG, and LDL-c of3D and3P group weresignificant higher than3Q group; The lee’s index, E2, P, leptin level, TC, HLDL-cand blood glucose concentration of3D group were not significant difference with3Qgroup.4. Group3D and Group3P compared with Group3U:Body weight and visceral fat weight of3D and3P group were not significantdifference with3U group; lee’s index and E2of3P group were significant higher than3U group, but no significant difference between3D group and3U group; The P of3Dgroup were significant higher than3U group, but3P group was not significantdifference with3U group; The leptin level, TC, TG, LDL-c, HDL-c and blood glucoseconcentration of3D and3P group were significant higher than3U group.5. Group3D compared with Group3P:Body weight, visceral fat weight, lee’s index, E2, P, TC, TG, HDL-c, LDL-c andblood glucose concentration of3D were not significant difference with3P group; Theleptin level of3P group were significant higher than3D group.6. Group6D and Group6P compared with Group C:Visceral fat weight and lee’s index of6D and6P group were significant lowerthan C group; Body weight, E2, P, leptin level, TC and HDL-c of6D and6P were notsignificant difference with C group; The TG of6D group were significant higher thanC group, but6P group was not significant difference with C group; The blood glucoseconcentration of6D and6P group were significant higher than C group.7. Group6D and Group6P compared with Group M:Body weight, visceral fat weight, E2, P, leptin level, TC, TG and HDL-c of6Dand6P group and6D group were significant higher than M group; The lee’s index andLDL-c were not significant difference; The blood glucose concentration of6D wassignificant higher than M group, but no significant between6D group and M group.8. Group6D compared with Group6P:Body weight, visceral fat weight, lee’s index, E2, P, leptin level, TC, TG, HDL-c,LDL-c and blood glucose concentration of6D and6P group were not significantdifference with6P group. 9. Correlation of Lipid metabolism index with Estrogen and progesterone:Visceral fat weight with Leptin, TC and E2was moderate positive correlation.Leptin with visceral fat weight and P was moderate positive correlation. TC withHDL-C and LDL-C was moderate positive correlation.Conclusion:1. Take carbohydrate to supplement female rat exogenous energy, rat weight andvisceral fat content increased, but lee’s index was significant lower than control group,the above shows that rats were not fat because of intake of sugar.2. Visceral fat content increased after supplement carbohydrate, which promoted thesecretion of leptin and the estrogen synthesis, reduced vaginal exfoliated cell number.Effectively relieve or prevent the estrous cycle inhibition.3. Supplement sugar for the estrous cycle inhibition rats, which lead increase of totalcholesterol and LDL-c, Provide enough raw materials for the synthesis of estrogen. Atthe same time, HDL-c was also increase.4. No significant difference between glucose and oligosaccharides on the role of lipidmetabolic of the estrous cycle inhibition... |
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