Effects Of Aerobic Exercise On ZAG Expression In Liver Of Rats With Insulin Resistance Induced By High Fat Diet

Objective: Insulin resistance(IR)model rats were established.The changes of ZAG protein and the level of mitochondrial biosynthesis regulator PGC-1? were measured before and after aerobic exercise to explore the molecular pathology of the level of IR in the body.To clarify the movement of mitochondrial metabolic pathway closely related to IR,and to provide theoretical reference for the prevention and control of IR.Methods: Forty 2-month-old Wistar rats were fed normally for 1 week and randomly divided into normal diet group(n = 20)and model group(n =20),modeling and feeding for 5 weeks.Model group were randomly divided into high fat no-exercise group(IR)and high fat exercise group(IRe).Normal group were randomly divided into normal no-exercise group(N)and normal exercise group(Ne).After the training of the adaptive runner,the second stage load of the Bedford program was used for the runner movement,5 times a week for 6 weeks.After 6 weeks of training,liver and blood samples were collected and subjected to Western blot and biochemical tests.Two-way analysis of variance was performed on the obtained data.Results:(1)In this study,according to the preliminary work of this group,the weight of rats during modeling,the results of high insulin and sugar forceps clip test and GIR coefficient of variation showed significant differences(P <0.05).Models were successful.The results of blood biochemical tests showed that after 6-week aerobics intervention,CHOL was1.576 ± 0.267 mmol / L in the NE group and 1.787?0.220mmol/L in the IRE group;the HDL-CHOL index in group NE was 0.437 ± 0.132 mmol / L,the HDL-CHOL index in IRE group was 0.353?0.096mmol/L.The LDL-CHOL index of the NE group was 0.310 ± 0.060 mmol / L,the LDL-CHOL index in the IRE group was 0.315 ± 0.0393 mmol / L.The TG index of the NE group was 0.427 ± 0.043 mmol / L,the TG index of IRE group was 0.752 ±0.050 mmol / L.The levels of CHOL,HDL-CHOL,LDL-CHOL and TG were significantly higher in N group and IR group than those in NE group and IREgroup(P <0.05).(2)The ZAG protein was 0.506 ? 0.014 in the N group,0.551?0.081 in the NE group,0.396?0.012 in the IR group,and 0.197?0.016 in the IRE group,N group and IR group were significantly lower than NE group and IRE group.N group and NE group,N group and IR group,IR group and IRE group were significantly different(P <0.05),NE group and IRE group were different,but no statistically significant.The expression of PGC-1? was 0.246 ? 0.021 in group N,0.265 ? 0.014 in NE group and0.157?0.067 in IR group and 0.196?0.022 in IRE group.N group and IR group were significantly lower than NE group and IRE group.N group and NE group,N group and IR group,IR group and IRE group were significantly different(P <0.05),NE group and IRE group were different,but no statistically significant.(3)According to the results of Western blot,the results showed that PGC-1? was positively correlated with the expression of ZAG and PGC-1? with the increase of expression of ZAG protein in liver tissue.Conclusion:(1)The expression of ZAG and PGC-1? protein in liver of IR rats induced by high fat diet was much lower than that of aerobic exercise in rats.The expression of ZAG and PGC-1? protein in rat liver showed that aerobic exercise could up-regulate the expression of ZAG Protein expression,lower blood lipids,improve lipid metabolism.(2)ZAG may promote the expression of mitochondrial biosynthesis-related factors in liver cells,and stimulate the mitochondrial biosynthesis-related signaling pathway in liver cells to enhance the effect of mitochondrial biosynthesis and improve IR.The expression of ZAG was negatively correlated with the levels of TG,CHOL and HDL-CHOL in the blood of rats,suggesting that ZAG may be a new candidate gene for regulating lipid metabolism.