| The experiment purpose:Aerobic exercise and experiment by old ratsmyocardial mitochondrial energy metabolism, the relationship between thecombination of free radical metabolism, aerobic exercise by looking at old rats model,the speed limit in the Krebs cycle enzymes: citrate synthase CS, isocitratedehydrogenase ICD, alpha ketone glutarate dehydrogenase (α-KGDHC), respiratorychain enzyme complexes (I, II, III and IV)and H﹢-ATP enzyme activity change ruleand free radical metabolism for aging mechanism to explain from the mitochondrialenergy metabolism aerobics anti-aging effect.The experimental method: Select and2and12months each20healthy femaleWistar rats were only40, animals run on adaptability training one week (0°,8.2m/min,5min/day) at first, formal training with moderate intensity (the equivalent of64%plus or minus4.5VO2max) slope,15.250m/min,15minutes for first day,2days30miutes for second day,45minutes for third day after, six days a week,12weeks intotal. Training for12weeks of rats after24hours out, placed in the ice tray caesareansection under anesthesia and remove the rats’ heart to determine citrate synthase CS,dehydrogenase ICD alpha ketone glutaric acid dehydrogenase activity,(α-KGDHC),respiratory chain complexes (Ⅰ、Ⅱ、Ⅲ and Ⅳ),H+-ATP enzyme activity, Mn+-SOD,GSH-Px, CAT activity and MDA.The experimental results(1)Aerobic exercise for aging rats myocardial mitochondria in the Krebs cyclespeed limit the influence of the enzyme activity observed in this study: MC15groupand the MC5group CS and reduction (α-KGDHC) activity but has no differencesobviously, ICD significantly reduced (P﹤0.05) has obvious differences afteraerobic exercises, the activity of MT15group is extremely study: the compound Ⅰof MC15and MC5group decreased significantly (P <0.05),complex Ⅱ and Ⅳ felldown for7.3%and14.5%,complex Ⅲ rose for6.6%, After aerobic exercise, Thecomplex Ⅰ、Ⅱ and Ⅲ of MT15and MC5hdifferent than the MC5CS.(P﹤0.05),(ICD) and (α-KGDHC) has no difference; The CS activity of MT15and MT5group is higher than the control group (P﹤0.01) and the ICD and the (α-KGDHC)(P <0.05) is different; (2)In aged rats with aerobic exercise on myocardial mitochondrial respiratorychain enzyme complexes Ⅰ to Ⅳ active effects were observed in thisas nosignificant difference, Activity of complex Ⅳ(P <0.05) has significant difference;The complex Ⅰof MT5group and MT15group is higher than the control group(P <0.01) The complex Ⅱ, Ⅲ and Ⅳ (P <0.05) has significant differences, and activity ofcomplex Ⅳ in MT5and MC5has no significant differences，the complex Ⅲ of MC15has not much difference than MT15, The complex Ⅰ, Ⅱ, Ⅲ and Ⅳ of MT15groupand MT5also has no significant differences.(3)The influence of aged rats with aerobic exercise on myocardial mitochondria(H+-ATP) enzyme activity were observed in this study:MC15is lower than MC5group for45.2%, after aerobic exercise: MT15and MC5group (P <0.05) hassignificant differences;MT5and MT15group is significantly higher than theirrespective control group;MT5and MC5has significant differences (P <0.05) andMT15group and MC15(P <0.01) has significant differences extremely, MT15andMT5group has no much differences.(4)Aerobic exercise in aged rats’myocardial mitochondria indicators related tometabolism of free radical were observed in this study: The activity (Mn+-SOD)ofMC15and MC5increased (P <0.05) obviously, GSH-Px activity decreased for2.7%,CAT activity decreased significantly (P <0.01), The MDA of MC15and MC5has noobvious difference, after aerobic exercise: Mn+-SOD,GSH-Px and CAT activity ofMT15and MC5increased obviously(P <0.01). Mn+-SOD of MT5and MT15issignificantly high,CAT of MT5and MC5is significantly high, the MDA in MC5andMT5has no obvious difference, and MT15group is MC15declined obviously andreduced for39.3%without significant differences. On the MT15the MT5group, Mn+-SOD activity and MDA is increased, and the CAT activity is reduced, they have nosignificant differences, GSH-Px activity (P <0.01) decreased and has significantdifferences.The experimental conclusion:(1)Aerobic exercise can improve the speed limit in aging rat myocardialmitochondria enzyme activity, further illustrate aerobic exercise training can improveaerobic oxidation power system and the Krebs cycle speed limit enzyme activity to improve the power capability.(2) In aged rats with aerobic exercise increases the activity of myocardialmitochondrial respiratory chain from Ⅰ to Ⅳ, for that the H+-ATPase activity ofenergy metabolism was strengthened.(3) Aerobic exercise can improve aging rats by antioxidant effect of myocardialmitochondrial energy metabolism, and improve the CAT,the activity of Mn+-SODand GSH-PX, to remove the effect of MDA, makes the cells from attacks by freeradicals, maintain the body’s movement. |
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