Changes Of Several Plant Hormones, Antioxidant Enzymes And Volatile Oil During The Germination Of Schisandra Chinensis Seeds

Schisandra sphenanthera Rehd. et Wils. is an important Chinese medicinal materials and also a protected wild medical material, which has the very high medicinal value. S. sphenanthera is mainly wild resource before the large market demand leads to sharp decline. Because of the difficulties it breed and the long dormancy, it has high value in ecologic and economic to study the mechanism of the seed germination and establishing a rapid germination technology. This experiment focus on its seed germination stage and study the physiology, plant hormones, antioxidant enzyme, and the volatile oil of to reveal the reason of S. sphenanthera seed germination in difficulties. The main results are as follows:1. The content of soluble sugar and soluble protein in the seeds of sand treatment 80 d and 110 d dormant met minimum, before starting the germination levels began to rise, when its highest levels also reached the highest germination rate in early stage of germination.2. During the dormancy of S. sphenanthera seeds, the content of GA3 sustained downward, which increased when the seed began germinat. The content of GA3 reached the lowest point in the end of germination. The content of ABA increased during the dormancy of seed. Before the seeds germinate, the content of ABA declined, then it reached the lowest point in the end of germination. The content of IAA stayed at lower levels during the dormancy. It reached the highest point before the beginning of germination, then it reached the lowest point in the end of germination. In addition, the value of GA3/ABA and GA3/IAA stayed at lower levels during the dormancy. The value started increasing before seeds germinate, it declined the lowest point in the end of germination.3. The antioxidant enzyme of SOD, POD, CAT activity met minimum before the seed germinat. The activity of SOD, POD, CAT grew largest when the fastest growth germination rate. In addition to the POD, SOD and CAT activity fell again in the end of germination. During the germination of S. sphenanthera seeds, the content of MDA rase in the first stage, and then decreased during the dormancy of seed, which rase again before the seed crack, and then sustained downward after the seed entered dormancy. The content of O2- increased by starting of sand treatment, which decreased in 90 d of sand treatment. The content of O2- reached the lowest level when the germination's rate increated the highest level, then it increased slightly, to the end of germination it fell to the lowest point.4. During the germination of S. sphenanthera seeds, the kinds and contents of germination inhibitors include of organic acids, aldehydes and so on, sustained high levels during the dormancy. During the germination of S. sphenanthera seeds, the kinds of terpenoid were less and the contents of terpenoid were lower during the dormancy, and the kinds and content of terpenoid increased at the start of germination, the kinds and content decreased in the middle of germination, and they decreased to the lowest in the end of germination.This experiment preliminary reveals the changes of every physical indicate during its seed germination in the natural environment in order to provide reference for the study of S. sphenanthera seed germination, to promote its seed germination and help improving seed germination rate, ultimately achieving the purpose of protect germ plasm resources of S. sphenanthera.