The Research On The Combining Ability Of The New Sterile Line Guifeng 105A And The Construction Of Fingerprint Map

sterile lines GuiFeng105 A is the main test materials in this test which is compound with24 crossing combinations according to the 3 × 8 NC?genetic way combined with other 2 sterile lines and 8 restorer lines.The growth characteristics and yield and agronomic traits of combining ability effect were investigated by joint identification at three sites.The results as follows:1.GuiFeng105 A vigorous plant,plant leaf shape is good,the upper middle tiller,stem stout,dark green leaves,leaf sheath,leaf margin,stigma,glume tips purple,a few grains have short awn.Flowering habit,pollen,completely stable sterility,fertility rate was 100%,and the flowering period is concentrated,spend time flowering ratio is high,high stigma exposure rate,different delivery performance is good,the equipping of hybrid combinations of difference in seed setting rate is 63.24% on average.2.The research about difference growth period of leaf chlorophyll content change by the 24 hybrid combinations found that the combination of chlorophyll content changes from high to low phenomenon,tiller peak stage> booting stage > filling stage > mature period;in the tiller peak stage,GuiFeng105 A combination of chlorophyll with the Qian209 A and Rong18 A that matched a basic average combination;between a booting stage and filling stage,GuiFeng105 A combination of chlorophyll were higher than Qian209 A and Rong18A;in the Mature stage,GuiFeng105 A combination of chlorophyll relative content is lower than Qian209 A and Rong18A;At the same time,GuiFeng105 A with different restorer equipping offspring of chlorophyll content slightly greater than the average Rong18 A and Qian209 A and same restorer lines equipping offspring average chlorophyll content;Three sterile lines combination offspring chlorophyll relative content are all characterized by the maximum in the tiller peak stage,a minimum value during ripening stage.3.This experiment from 50 to extract the RM1254 SSR primers,RM493,RM5699,RM3688,RM3874,RM3474,RM3317,RM1221,RM6909,RM1186,RM1279,RM6382,RM1615,RM524,RM6288,RM1337 by a total of 16 primer used to construct the genetic linkage maps.4.The research about GuiFeng105 A sterile lines combinated the nine major agronomic characters variability analysis found that GuiFeng 105 A combination with the coefficient ofvariation range between 1.24% and 6.71%,its variability is stable,the characters and variation coefficient of size for: the total number of grains per ear > panicle effective ear > yield per spike >plant height > ear length > setting in the whole growth period;Thecorrelation analysis with factor arousing fromGuiFeng105 A yield and yield components found that your abundant output of GuiFeng105 A and growth period,plant height,effective spike,spike length were positively correlated,the effective panicles was significantly positively related;And the total grain number per spike,panicle,seed setting rate,grain showed a negative correlation,the correlation coefficient of size: panicle effective ear > the total number of grains per ear > ear length > setting in the whole growth period effective ear > plant height > seed setting rate > panicle effective ear;In the whole growth period?X₁?,plant height?X₂?,effective spike?X₃?,ear length?X₄?,the total number of grains per ear?X₅?,panicle?X₆?,seed setting rate?X₇?,grain?by 8?as the independent variable,by stepwise regression analysis.Establishment and yield?Y?the optimal Y=-911.60+58.77X₃-20.98X₄-1.142X₅+2.51X₆+30.68X₈,equation were selected six independent variables had a significant effect on yield,effective spike?X₃?,ear length,respectively?X₄?,the total number of grains per ear?X₅?,panicle?X₆?,grain?X₈?;On the basis of regression analysis,for your abundant 105 a combination with path analysis,the yield and yield components do further found that six agronomic traits to yield?Y?direct path coefficient of the size of the sequence is: the panicle?X₆?> total number of grains per ear?X₅?> seed setting rate?X₇?effective ear?X₃?> grain?X₈?> ear length?X₄?,including panicle,seed setting rate and effective panicle,ear length for direct positive effect,total grain number per spike and show the negative effects5.The analysis variance of GuiFeng105 A pilot 24 hybrid combinations with combination of three main agronomic traits of showed that 24 hybrid combinations in the same site,three different block various characters in addition to yield significant differences exist significant difference and seed setting rate,other traits were no difference;The same cross combination between three different sites,effective spike,grains and per panicle reached significant or extremely significant difference;Different cross combinations between spike length,effective spike,total grain number per panicle,seed setting rate and grain of extremely significant difference;Testing points of the different combination in different plant height,spike length,effective panicle,solid grains per panicle,seed setting rate,grain and yield were extremely significant difference;Differentcombination between varieties and pilot interactions in addition to the plant height,yield and effective spike did not reach significant difference,the rest of the various characters are reached extremely significant level.6.Agronomic traits NC ? mating design combining ability variance analysis results showed that the combination of 24 studies eight characteristics of the combination between differences were very significant level,these differences mainly controlled by genetic traits.Combining ability variance analysis result between combination and sterile lines by combining ability variance of restorer lines were reached significant level.Sterile lines of general combining ability variance in spike length,seed setting rate,grain three properties reached extremely significant level,the effective ear,per panicle,total grain number per panicle and yield reached significant level;Restorer of general combining ability variance in spike length,per panicle,total grain number per panicle and grain traits in reached extremely significant level,the plant height and seed setting rate and yield traits in reached significant level.7.The analysis of 11 parent material showed that general combining ability effect in the same characters,and there are significant differences among parents,which suggested that the parent on the different characters of the additive effect size is different,and the differences between genetic traits.Your abundant 105 a in spike length,seed setting rate,grain and yield three agronomic traits general combining ability relative effect has the highest value.Illustrated the better comprehensive economic characters of sterile lines,general combining ability is strong,which makes the combination of seed setting rate is high,the more large grains,ear length is long,etc.8.The 24 hybrid combinations eight agronomic traits of special combining ability?SCA?analysis showed that hybrid combinations in the same combination of different characters in different special combing ability?SCA?;For the same characters,the same female parent sterile lines and male parent restorer lines cross combinations of equipping the special combining ability effect value is different;For the same characters,the same parent restorer lines and different maternal sterile lines cross combinations of equipping the special combining ability effect value is not equal.In short,with combination performance by GuiFeng105 A better comprehensive performance compared all 24 portfolio,multiple composite performance in different traits.Show GuiFeng105 A is a good combining ability of sterile lines.9.The analysis relied combinations of 8 agronomic characters of 24 at three sites of specialcombining ability?SCA?found that the same hybrid combinations in different sites of SCA there are certain differences in both size and direction under the same character.Moreover,For the same combination of SCA exist differences between different characters in the same place,also vary in different locations.Different combination in the same site SCA is different,in different sites are also different.10.In this test,the result of the heritability analysis showed that GuiFeng105 A in addition to the productive ear and total grain number per panicle,all properties of the generalized heritability were all above 50%;the narrow sense heritability analysis results showed that ear length,the total number of grains per ear,panicle,seed setting rate and effective panicle narrow sense heritability of five properties such as low.8 agronomic traits is broad sense heritability from big to small order:plant height > yield per spike > ear length > per panicle > seed setting rate > total grain number per panicle > effective panicle;The narrow sense heritability by to small order greatly for: plant height > yield > total grain number per panicle > ear length > per panicle > seed setting rate effective ear >grain.