Effect Of Huoxuerongong Tablet On The Expression Of AchE And ChAT In AD Model Mice

Objective:To investigate the effect of the blood circulation and the tablet on the expression of cholinergic related protein in brain tissue of AD mice. From the perspective of cholinergic system, the possible therapeutic effect of promoting blood circulation to the treatment of Alzheimer's disease is discussed, and it can provide some experimental evidence for its clinical application.Methods:50 app/PS1 double transgenic AD mice randomly divided into model group, blood activating Rong luopian low dose group, Huoxue Rong complex film dose group, promoting blood circulation and Rong luopian high dose group, donepezil group,10 rats in each group. Over 10 KM mice were the same age as the normal group. Blot AchE was used to detect the Western and ChAT contents in the brain of mice.Results:1. Nissl staining:model group, neurons damage larger, invigorate the circulation of blood Rong collaterals tablet group and donepezil group mouse neural cell number compared with the model group, have varying degrees of increase, more uniform distribution, structural integrity.2. Western Blot:1) compared with the normal group, model group mice brain organize ache protein expression were significantly increased (P< 0.01); compared with the model group, high dose of low blood Rong collaterals tablet group, donepezil group of brain AChE protein expression decreased (P< 0.01); compared with donepezil group, low dose of live blood wing luopian group brain organize ache protein expression increased (P< 0.05), and middle and high dose of Huoxue Rong luopian group AChE expression changes had no statistical significance (P> 0.05).2) compared with the normal group, model group mice brain chat protein expression were significantly decreased (P< 0.01); compared with the model group, low and high dose Huoxue Rong collaterals tablet group, donepezil group of brain tissue chat protein expression increased (P< 0.01); and donepezil group compared low, middle dose of Huoxue Rong luopian group brain chat protein expression changes had no statistical significance (P> 0.05), and high dose of Huoxue Rong luopian group chat expression increased (P< 0.05).Conclusion:1 the injury of hippocampal neurons in the APP/PS1 transgenic mice was reduced by the Huoxue tablet group.2 huoxuerongluo pill group could inhibit the AchE protein expression in the brain tissue of the body.3 huoxuerongluo pill group can promote the expression of ChAT protein in the brain tissue of the body.