From The Perspective Of HPG Axis, The Mechanism And Pharmacodynamic Components Of Liuwei Dihuang Decoction And Its "reinforcing And Diarrhea" Drugs Were Discussed

Objective To investigate the effect mechanism of nourishing yin and supplementing kidney of Liuwei Dihuang Decoction and “reinforeing and reducing” herb couples on hypothalamus-pituitary-gonad(HPG)axis in neuroendocrine system.To compare Liuwei dihuang decoction and its best “reinforeing and reducing” herb couples with the content of the main component of them.It's main to clarify the effect mechanism of supplementing kidney,the main components and the principle of compatibility.MethodsThe mices were randomly divided into six experimental groups: blank group,model group,Liuwei Dihuang Decoction group,Cornus officinalis-Cortex moutan couple group,Prepared radix rehmanniae-Alisma orientale couple group and Chinese yam-Poria cocos couple group.The rats were gavaged with oral administration of corresponding medicines for 9 days.At 6-9d,in addition to the blank group was given distilled water,the other groups were gavaged with hydrocortisone injection per day according to 50 mg/kg body weight.To observe the survival status of the mice in each group and the weight was measured.After 9 days,the contents of cyclic adenosine monophosphate(c AMP),cyclic guanosine monophosphate(c GMP)and follicle-stimulating hormone(FSH),estradiol(E2),testosterone(T)were determined by ELISA and radio immunoassay,gonad was observed.To separate and determine the content of sweroside,paeoniflorin and loganin in Liuwei dihuang decoction and “Cornus officinalis-coxtex moutan”herb couple by RP-HPLC.The HPLC method was used at Hypersile C18column(4.6 mm×250 mm,5?m),the mobile phase consisted of methanol-water(24:76),the detective wavelength was 236 nm,the flow rate was 1.0ml/min,the column temperature was 30?.Results1.Compared with the blank group,the kidney-yin deficiency mice model group associated with weight loss(P<0.01),the plasma c AMP was increased,while plasma c GMP and serum FSH,E2,T contents in HPG were decreased,the difference was statistically significant(P<0.01).2.Compared with the modle group,Liuwei Dihuang Decoction group could increase the weight,decrease the plasma c AMP,increase the plasma c GMP contents and serum FSH,E2,T contents in HPG axis(P<0.05,P<0.01)and the gonads(testicles and uterus)index(P<0.05),the damaged gonad tissue were repaired.3.Compared with the modle group,Cornus officinalis-Cortex moutan couple group could decrease the plasma c AMP,increase the serum FSH,E2,T contents in HPG axis(P<0.05,P<0.01),and the damaged gonad tissue were repaired.4.Cornus officinalis-Cortex moutan couple mainly contains sweroside,paeoniflorin and loganin,and the calibration curves of them were respectively linear in the range of 0.10?g~1.65?g(r = 0.9995),0.12?g~1.92?g(r = 0.9991),0.23?g~3.36?g(r =0.9997).The recovery and relative standard deviation of them were 102.29%,100.99%,102.48% and 1.48%,1.32%,0.75%.The average content of sweroside,paeoniflorin and loganin to the material were 1.48%,1.32%,0.75%.And the average content of them in Liuwei Dihuang Decoction were 0.081%,0.104% and 0.363%Conclusion1.To gavage with oral administration of hydrocortisone as 50 mg/kg for 4 days can establish kidney-yin deficiency mice model successfully,the plasma c AMP,c GMP contents and serum FSH,E2,T contents could be used as an important index to evaluate the model of kidney-yin deficiency by glucocorticoid.2.Liuwei Dihuang Decoction could improve kidney-yin deficiency syndrome,and its mechanism may possible to decrease the content of plasma c AMP,increase plasma c GMP content,FSH,E2,T content in HPG axis,the uterus and testis index,and repair the damaged the uterus and testis organizations.The Cornus officinalis-Cortex moutan couple and its sweroside,paeoniflorin and loganin could be the efficiency herb couple and main components in Liuwei Dihuang Decoction.