Anti-inflammatory Effect And Mechanism Of Astragalus Charcoal

Background:The dry roots of Scutellaria baicalensis georgi,a yellow plant in the labiatae family,are bitterly cold.They were first introduced in the "Shen Nong's Herbal Classics" and have the effects of heat the dampness,purging and detoxification,hemostasis,and tocolysis.Scutellariae Radix(SR)is widely used in the study,and there are 25 cases of scutellaria in Treatise on Febrile and Miscellaneous Diseases.The modern clinical commonly used prescriptions contain SR for the treatment of a variety of inflammatory and immune diseases.SR may slow down its medicinal properties after carbon making.Although the power of heat is slightly weakened,it does not hurt Zheng,and the effect of convergence is obviously enhanced.Current research on the active ingredients and mechanism of action of carbon drugs is still in a state of confusion.In this paper,taking advantage of modern nanomaterials research methods,a new way of methods has been used to explore the anti-inflammatory effects of Scutellariae Radix Carbonisatus(SRC).Purposes:1.The carbon dot of SR was isolated from Radix Astragali charcoal,and the difference of charcoal carbon do of SR at different temperatures was compared to optimize the preparation temperature.2.Detailed characterization of charcoal carbon nanoparticles in SRC.3.Establish an experimental animal model of inflammatory response to clarify the anti-inflammatory effects of SRC carbon nanoparticles.4.Establish a model of cell degranulation,explore its anti-inflammatory mechanism,and extend its new role.Methods:1.Study on the optimization of the separation and preparation temperature of charcoal carbon nanoparticles of Scutellaria baicalensis Georgi:preparation of Scutellaria baicalensis charcoal at different temperatures,separation and combination of charcoal drug yield,carbon spot morphology,physicochemical characteristics,and the like by means of filtration,dialysis,etc.With the comprehensive comparison of biological effects,the optimal preparation temperature is optimized to provide the next step of research.2.Characterization of charcoal carbon nanoparticles in SRC:Using nanomaterials research techniques,the charcoal carbon nanoparticles composition of purified SR was identified.High-resolution transmission electron microscopy(HR-TEM)and X-ray photoelectron spectroscopy(XPS)were used.Fourier transform infrared spectroscopy(FT-IR)and other analytical methods provide detailed characterization of carbon nanoparticles in SRC.3.Study on the anti-inflammatory effect of SRC carbon nanoparticles:The experimental animal model of xylene induced ear swelling and acetic acid induced peritonitis was used to compare the ear swelling rate and Evans blue exudation volume in abdominal cavity.The anti-inflammatory effects were evaluated.4.Anti-inflammatory effect and mechanism of SRC carbon nanoparticles:The CCK-8 was used to detect the cytotoxicity of carbon dot in SRC,and it was possible to use the RBL-2H3 cell degranulation model to exclude its cytotoxicity by cytotoxicity.The effect of inhibiting cell degranulation was evaluated by cell morphology;the degree of histamine,?-hexosaminidase,IL-?,IL-6,and TNF-a in cell supernatants was measured by which the mechanism of anti-inflammatory action was initially investigated.Results:1.Study on the optimization of the separation and preparation temperature of charcoal carbon nanoparticles in SRC:The results showed that different temperature preparation of SRC carbon nanoparticles each has its characteristics on the morphological and optical characteristics,among them the charcoal yield of SRC at 280? was the highest,and more obvious biological effects than other preparation temperature.2.Characterization of charcoal carbon nanoparticles in SRC:The carbon particle size distribution of SRC is in the range of 2-9nm with a lattice spacing of 0.335nm.It is mainly composed of carbon and oxygen.Its surface groups are abundant and obvious shoulders appear at the ultraviolet 278nm..3.Study on the anti-inflammation effect of SRC carbon nanoparticles:SRC carbon nanoparticles has a strong inhibitory effect on xylene-induced acute ear swelling,it can reduce the permeability of capillaries in the abdominal cavity of acute peritonitis mice.4.Anti-inflammatory effect and mechanism of SRC carbon nanoparticles:SRC carbon nanoparticles has a stable biological safety,which means that its anti-inflammatory effect is not due to cytotoxicity.It has a significant inhibitory effect on RBL-2H3 cell degranulation.The content of histamine,-hexosaminidase,IL-1?,IL-6 and TNF-a in the supernatant of the cells were decreased compared to the model control group.Conclusion:In this experiment,the carbon nanoparticles was isolated from the Chinese traditional medicine SRC and characterized in detail.Then the anti-inflammatory effect of SRC carbon nanoparticles was determined using different experimental animal models.The anti-inflammatory mechanism was initially studied using cell in vitro model.It is closely related.to its role in stabilizing mast cell activation,possibly by downregulating the activation of mast cell function,inhibiting its degranulation,and reducing the effect expression of histamine and inflammatory mediators(IL-1?,IL-6 and TNF-?).The above conclusion provides the basis and new ideas for the clinical application of SRC,and also provides more development space for the clinical application of TCM classic prescriptions.