Effects Of Aerobic Exercise On Brain Aging, Learning And Memory Ability And Expression Of BDNF In Frontal Lobe In Rats At Different Stages

Purpose: this study by observing the aerobic exercise on SD rat model of spatial learning and memory ability of ageing and in the frontal lobe brain derived neurotrophic factor(BDNF)expression and the effect of aerobic exercise on the effects of aging rats and the mechanism of action.Methods: 3 months,a total of54 male SD rats,randomly divided into 6 groups: normal saline injection control group(NNa),D-galactose injection to aging group(ND),D-galactose injection to aging before exercise groups(SD),D-galactose injection to the process of ageing in the exercise group(NDs),D-galactose movement before and after the injection to aging group group(NDs),and D-galactose don't exercise group after injection to aging(NDN),each group of nine(n = 9).SD group for 4 weeks prior to the aging building aerobic exercise intervention,NDs group on aging in the process of building6 weeks aerobic exercise intervention,NDs group was performed after the aging building 4 weeks aerobic exercise intervention,NDN group does not exercise intervention after the old building.Selects the Morris water maze training system and examine each group rats navigation and space exploration ability.After the experiment,beheaded kill,separation of frontal lobe in rats.Application of immunohistochemical,Real time PCR and Westren-Blotting technology respectively to detect the frontal BDNF gene mRNA and protein expression.Results: 1.The testing results of free radicals,ND than NNa group activity of gsh-px and SOD increased significantly(P < 0.05),MDA content decreased significantly(P < 0.05);NDs group than a ND the activity of gsh-px and SOD increased significantly(P <0.05),MDA content decreased significantly(P < 0.05);SD group than a ND the activity of SOD,gsh-px and MDA content were not significant difference(P > 0.05);NDS group than NDN gsh-px and SOD activity increased significantly(P < 0.05),MDA content decreased significantly(P < 0.05);NDN group than a ND the activity of gsh-px and SOD has not significant difference(P > 0.05),the content of MDA increased significantly(P < 0.05);NDs than NDs group significantly increased the activity of gsh-px and SOD(P < 0.01),MDA content decreased significantly(P <0.01).2.The testing results of spatial learning ability:(1)platform across a number of times: ND group than NNa platform through the original time significantly reduced(P< 0.05);NDs than ND group increased significantly(P < 0.05),between SD and ND group was no significant difference(P > 0.05);NDN with NDS group is not significant difference(P > 0.05).(2)the original platform quadrant swimmingdistance and the ratio of total: ND group was significantly lower than NNa group(P <0.01);NDs group was significantly higher than that of ND group(P < 0.01),SD and ND group no significant difference(P > 0.05);NDN with NDS group has no significant difference(P > 0.05).3.The BDNF expression in: ND group is significantly lower than the number of BDNF protein expression NNa group(P <0.05);NDs group than BDNF mRNA and protein expression of ND group were significantly lower(P < 0.01),the SD group of BDNF mRNA and protein expression quantity compared with ND group was no significant difference(P > 0.05);NDS group of BDNF mRNA and protein expression and NDN group is not significant difference(P > 0.05).Conclusion: 1.Through six weeks of D-galactose injection successfully build the subacute aging rat model.2.The brain aging process of aerobic exercise intervention can improve the ability of learning and memory of rats,increase the frontal the expression of BDNF,aerobic exercise intervention in aging process to delay the aging brain.3.The frontal BDNF protein expression is linear positive correlation with learning and memory,the increased amount of expression is one of the mechanism to improve the ability of learning and memory.