The Effect Of Aerobic Exercise On Learning And Memory Ability And Cerebellar NCAM Expression In Rats During Aging

Objective: The aging of SD rats induced by D-galactose was subjected to aerobic exercise during aging,to investigate the effect of aerobic exercise on spatial learning and memory ability and cerebellum NCAM gene expression.Methods: Choose 36 male SD rats of 3-month-old were randomly divided into 3groups(n=12)and adaptive feeding for 7 days.Three groups were named as normal saline control group(group C),D-galactose-induced aging model group(group D),D-galactose induced senescence and aerobic exercise intervention group(DS group),After the group were 6 weeks of aging modeling,followed by each group were randomly divided into two groups: One group of natural feeding for 7 days(N)and the other for 7 days of Morris water maze test(M),respectively,labeled CN,DN,DSN,CM,DM,DSM,a total of 6 groups(n=6).The rats were treated with D-galactose(100mg/kg/d)for 6 weeks.The injection site was abdominal cavity.Morris water maze system was used to determine the effect of aerobic exercise on spatial learning and memory in rats.The activity of SOD and GSH-Px and the amount of MDA in the cerebral cortex of each group were measured to determine the senescence of the rats in each group.Real-time PCR and Western blotting were used to detect the expression of NCAM in cerebellum of rats.Results:(1)Rat state observation: Compared with group C rats,D group of rats mental state is poor,there are apathetic,lethargy,reduced diet,poor fur elasticity,dull and hair removal and other aging characteristics;(2)Morris water maze positioning flight test results: In the course of positioning navigation experiments,the average escape latency of each M group rats gradually decreased with the number of training to stabilize,but different groups between the average escape period with a certain difference.The mean escape latency of all groups was significantly less than that on day 1(P<0.01).The mean escape latency of the rats on day 3 was significantly less than that on day 2(P<0.01).The mean escape latency of CM group and DSM group was stable from the third day(P>0.05).While the DM group was stable at the 4th day of the mean escape latency(P>0.05).The aim of DM group was significantly higher than that of CM and DSM group(P<0.01).The results showed that the average number of CM rats crossing the original site was significantly higher than that of the DM group(P<0.01).Compared with the DM group,the number of DSM group in the DSM group was significantly higher than that in the DM group(P<0.01).There was no significant difference between the CM group and the DSM group(P>0.05).(3)Free radical test results:There was no significant difference in MDA content and SOD and GSH-Px activity between group M and corresponding N groups(P>0.05),and the trend was similar.Compared with DN group,the content of MDA in the cerebral cortex of CN group was significantly decreased(P<0.01),and the activities of SOD and GSH-Px were significantly increased(P<0.01).Compared with DN group,the content of SOD and GSH-Px in DSN group were significantly lower than those in DN group(P<0.01).There was no significant difference in SOD,GSH-Px activity and MDA content incerebral cortex of DSN group(P>0.05).(4)The expression of NCAM m RNA in cerebellum was not significantly different from that in group N(P>0.05),and the expression of NCAM was similar.Compared with group D,the expression of NCAM mRNA and protein in cerebellum of group C was significantly higher than that of group D(P<0.01).The expression of NCAM mRNA in cerebellum of DS group was significantly higher than that in group D(P<0.05)Protein expression was significantly increased(P<0.01).Conclusion:(1)D-galactose-induced aging rats were successfully modeled.(2)Aerobic exercise in the process of aging to delay the process of brain aging in rats have a very significant role in promoting.(3)Aerobic exercise in the process of aging on the spatial learning and memory ability of rats significantly improved.(4)It is speculated that aerobic exercise is one of the mechanisms of delaying the decline of spatial learning and memory in rats by up-regulation of cerebellum NCAM expression.