Research On Honeybee Cybor Based On Embedded Optic Lobe Control

Honeybee has excellent flight continuity and maneuverability.The special system that uses MEMS(micro electrical mechanical system)to control the bee’s movement and then uses its biological ability is called cyborg honeybee.Electrical stimulation of the optic lobe is one of the effective control methods for cyborg honeybee,but its neural mechanism research and miniaturized knapsack design are still faced with great difficulties.In this paper,cyborg honeybee(Apis mellifera ligustica)is taken as the research purpose,including the study of behavior regulation mechanism and the design of light MEMS.Finally,the flight control of cyborg honeybee is verified by using honeybee abdomen as the combination position of honeybee and machine.Aiming at the control mechanism of cyborg honeybee,the visual evoked potential(VEP)was found in the optic lobe of honeybee for the first time through the electrophysiological experiment,which responded positively and negatively to the change of light intensity,and the amplitude of VEP was positively correlated with light intensity.Based on the calcium ion fluorescence imaging experiment,it was found that the neural network of optic lobe had stronger electrophysiological activity under light.In order to reveal the response mechanism of optic lobe to light,the potential response model was constructed by introducing the theory of neuron ion activity.Through the analysis of the model,it was found that electrical stimulation can affect the release of visual neuron action potential,thus causing visual illusion and inducing bees to fly.Aiming at the design of light MEMS system,infrared communication and PIC microcontroller were used as the main body to build the whole system.First,different modules were selected based on the factors of structure,function and weight,and the initial scheme was optimized through module splitting,introduction of new paper battery,and application of printing electronic technology,so as to reduce its weight to within the bee load.Then,the circuit principle and integrated circuit were designedbased on the principle of line optimization and area minimization.At the same time,the infrared decoding program was written by using MCU interrupt and NEC protocol,and the wireless variable parameter stimulus output was realized by cycle recognition.In order to verify the flight control,we use the abdomen of honeybee as the fixed position of MEMS to design cyborg honeybee.According to the flight start experiment of bilateral electrode output,the highest success rate of stimulation parameters with frequency of 100 Hz is 33%(N = 27).The flight steering experiment with unilateral electrode output showed that the direction of bee steering was always opposite to the direction of electrical stimulation and the success rate of induction was72%(N = 18).The experimental results preliminarily demonstrate the rationality of the scheme and the feasibility of cyborg honeybee trajectory control by single and double side stimulation.