Effects Of Different Fasting Time On Intestinal Flora Of Healthy Mice

The human microflora consists of bacteria,archaea,viruses,and single-celled eukaryotes,representing 1,014 microbial cells/humans that live peacefully in our bodies.These microbes are found in our skin,genitourinary system,respiratory tract and gastrointestinal tract.Human body is a superorganism composed of its own cells and microorganisms.These microorganisms reside in corresponding parts,form relatively stable communities,and interact with the human body to form a united body with symbiotic relationship,which is called a microecosystem.The study of intestinal microecology is mainly about the relationship between the quantity,species,composition and metabolites of intestinal microorganisms and diseases.There are 1,000 to 1,150 species of 100 trillion bacteria in the human gut,10 times the number of human cells,and a small number of fungi and viruses.There are a wide variety of microbes in the human gut,including bacteria,archaea,fungi and viruses.Among them,bacteroidetes and firmicutes are the main components,and actinomyces,proteus and verruca are the secondary components Human health is closely related to intestinal flora.Through interaction between host and intestinal flora,intestinal flora is in ecological balance.Imbalance of intestinal flora caused by various reasons will affect human health and lead to various diseases.Therefore,it is of great significance to study the relationship between intestinal microecology and diseases of various organs and systems.Intermittent fasting(IF)is the practice of fasting without food for 12 to 24 hours.During the fasting period,all food and water intake is prohibited,relying only on energy storage in the body to protect the needs of life activities,in order to achieve the purpose of treatment or prevention of a disease.Dietary interventions with intermittent fasting contribute to the health of the intestinal flora,but this effect may be influenced by the exact fasting regimen.The purpose of this study was to evaluate the effects of different fasting times on the formation of intestinal flora in mice.Healthy C57BL/6J male mice fasted for 12,16,or 20 hours per day for one month,then allowed all mice to eat freely for an additional month.Intestinal microbiota was analyzed by 16 S rRNA gene sequencing and feeding conditions were recorded.The differences of diet,body weight and intestinal flora environment of mice in each group were observed by comparing the stage of dietary restriction and the stage of dietary restriction.The research results are as follows:1.There was no significant difference in food intake for all mice during fasting.After resuming the free diet,all the mice were in the free diet stage,and the food intake would increase significantly until 60 d.There was no significant difference between the food intake of all the mice and that of the mice that had just fasted.Mice in the 16h/d and 20h/d fasting groups had stable food intake at this stage of the trial.2.Reducing food intake did not result in weight loss during the restriction period.After resuming the free diet,the new stable value of body weight also appeared,but the new stable value was not much different from the stable value of the fasting stage of the mice at 30 days.The body weight of the mice in each group remained relatively stable throughout the trial period.3.The 16h/d fasting regimen resulted in a significant increase in the level of Akkermansia and a decrease in the level of the genus Alistipes.The fasting pattern of 16h/d led to more significant correlation and more clustering in the flora network.4.Indirect fasting has a significant effect on mice.When normal diet is resumed,all the indicators of mice will recover to a certain extent with the disappearance of the influence.Due to the difference in the duration of fasting,the significant effect of the recovery is different to some extent.Combining all the data,the comparative analysis found that the fasting duration of 16h/d was relatively healthy,and the intestinal environment of the mice was maintained at a healthy level after the normal diet was resumed.In conclusion,the comparative analysis showed that the 16h/d fasting regimen was relatively healthy.After the normal diet was resumed,the intestinal environment of the mice was maintained at a healthy level.Therefore,the results of this study give a satisfactory result to our original intention of designing the experiment.But the study was conducted on a small group of animals for a short period of time.Therefore,further research is needed to determine the best fasting regimen to provide long-term beneficial results.