Preparation Of Lvcium Ruthenicum Murr Instant Powder And The Quality Evaluation

In order to increase the convenience and efficiency of using Lycium ruthenicum Murr and to broaden its application,Lycium ruthenicum Murr instant powder was prepared by ultrasonic-assisted extraction and spray drying technique.Then its quality and bioactivity were studied.In addition,to learn more knowledge about the structure and function of anthocyanins from Lycium ruthenicum Murr and its related product,anthocyanins purification process was studied.The extraction process was optimized through orthogonal test.The methods to dry aqueous extract(hot air drying,ultrasonic drying,freeze drying and spray drying)were compared.Spray drying process was optimized by using response surface method.In addition,the micro morphology,particle size,and dissolution time of the obtained instant powder were studied.Ultrasonic extraction results showed that the optimal extraction conditions were as follows:ultrasonic power 400 W,solid-to-solvent ratio 1:20(g/mL),extraction temperature 20?.,extraction time 50 min.The optimal spray drying technological parameters were 190?,20 mL/min and 1.01 for inlet air temperature,inject speed and liquor density,respectively.The range of size distribution of powder produced by spray drying was 4?60 ?m and the mean particle size was 17?m.The average dissolution time of instant powder was 43.4 s,which reached the industry standard.The grade,nutritive compositions and bioactivity of instant powder were measured and analyzed.The instant powder is Grade A and 17 kinds of amino acids were determined with the highest concentration of aspartic acid(1.345%).The contents of total flavones,procyanidins,polysaccharide and anthocyanins in instant powder were measured using sodium nitrite-aluminum nitrate method,vanillin-concentrated hydrochloric acid method,phenol-sulfuric acid method and pH differential method,respectively.Results showed the contents were 6.02%,2.88%,6.88%,2.88%,respectively.The HPLC-DAD analysis showed that there were a variety of anthocyanins in the instant powder,and two of the main anthocyanins were pelargonium-3-o-diglycosides and the pelargonium-3-O glucoside.Instant powder had strong reducing power and can significantly eliminate ABTS+ and DPPH free radicals.In ABTS+ and DPPH radical scavenging assays,the free radical scavenging rate was 50%when instant powder concentrations were 0.077 mg/mL,0.061 mg/mL,respectively;These results support strong antioxidant activity in vitro for instant powder.Extraction technology,macroporous resin,glucan gel and semi-preparative chromatography were used in the separation and purification of anthocyanin from Lycium ruthenicum Murr.After water extract was extracted with ethyl acetate three times,the ethyl acetate phase was close to colorless.The best adsorption macroporous resin(AB-8),optimal sample volume(6 BV)and optimal eluent condition(40%ethanol solution)were determined by static adsorption experiment,dynamic adsorption leakage experiment and dynamic elution experiment,respectively.Two Lycium ruthenicum Murr anthocyanin monomers were obtained when the sample purified by AB-8 macroporous resin was processed by glucan gel LH-20 and semi-preparative liquid chromatography.The effects of Lycium ruthenicum Murr instant powder,crude polyphenols and crude polysaccharide on the sleep quality of mouse were studied.The results showed that:the three samples had no significant effect on barbital sodium induced mice sleep time,however can reduce the number of autonomic activities of mouse;All three samples could reduce the serum MDA level and increase serum SOD activity of the mouse;High dose of Lycum ruthenicum Murr crude polyphenols could shorten the sleep latency of mouse.