Formulation Of Resveratrol W/O/W Delivery System And The Mechanism Of Its Absorption And Transport

Resveratrol,as a natural polyphenol,has many physiological functions such as anti-cancer,antibacterial,anti-inflammation,and estrogen regulation.However,due to its unstable chemical properties and poor water solubility,there are disadvantages such as poor oral absorption,low bioavailability,and difficulty in sustaining effects.The W/O/W emulsion has double oil-water interface membranes,which can prolong the release time of emulsified components compared to monolayer emulsions,thus it has a better protective effect on resveratrol.And it can be used in medicine,food,cosmetics and other industries.In this paper,the preparation process of resveratrol W/O/W emulsion was studied.The resveratrol W/O/W emulsion system was prepared by high pressure homogenization method.The physicochemical properties and stability of the resveratrol emulsion were analyzed,and the mechanism of W/O/W emulsion also be studied.The main conclusions are as follows:The resveratrol W/O/W emulsion was prepared by high-pressure homogenization method.Different W/O/W emulsion was prepared by using different lipophilic emulsifiers,different hydrophilic emulsifiers,different the ratio of oil phase and internal aqueous phase,different internal and external phase homogeneous pressure.And then its potential,microstructure,entrapment efficiency and zeta potential were measured to obtain the best preparation process of resveratrol W/O/W emulsion.And under this condition,the encapsulation efficiency of the emulsion was determined to be 99.97±0.001%.The stability of emulsion at different pH?2 to 8?,different NaCl concentrations?0 to 500 mM?,different temperatures?25? to 70??and different heating times?0 to 2 hours?was compared.The microstructure of the emulsion,particle size,zeta potential were analyzed.The emulsion is unstable at pH=2 and but when the pH 3 to 8,the emulsion has good stability;the emulsion is unstable under the presence of NaCl;with the increase of heating temperature and heating time,the stability of the emulsion becomes worse and worse.The in vitro digestion model was used to study the digestibility of the emulsion.The particle size,zeta potential and microstructure of the emulsion were analyzed.It was found that the emulsion has good stability in simulated oral and simulated gastric fluid environments.The digestion of the emulsion occurs mainly in the simulated intestine.Using Caco-2 monolayer cells as a model to study the absorption and transport mechanism of the resveratrol emulsion.Ultra-high performance liquid chromatography was used to analyze the content of resveratrol.According to the ratio of P_app?BL-AP?/P_app?AP-BL?is between 0.5 of 1.8,it can be known that the emulsion is passive diffusion in the body.