The Protective Effect And Mechanism Of Chlorogenic Acid On The Intestinal Barrier Of Cadmium Exposed Rats

Cadmium is a highly toxic heavy metal that is one of the most toxic substances that accumulates in the body..Exposure to cadmium can cause liver,kidney,gastrointestinal,reproductive,bone,and cardiovascular diseases.Among them,intestinal absorption is the most important way for cadmium to enter the body,so the intestine also becomes a target organ for cadmium toxicity.Previous studies have shown that cadmium exposure can cause stress in the intestine and destroy the intestinal barrier,while chlorogenic acid has antioxidant,antibacterial,antiviral,antitumor,blood pressure,blood fat,free radicals,and protection.Intestinal barrier and immune regulation.In this experiment,the chlorogenic acid in sunflower kernels was firstly extracted,then the cadmium exposed rats were used as a model,and chlorogenic acid was intervened to study the chlorogenic acid-induced cadmium exposed rats'growth performance,intestinal antioxidant function,and barriers.The protective effect and the effect on the absorption of cadmium.The effect of water bath temperature,time,solid-liquid ratio and ethanol concentration on the yield of chlorogenic acid was examined by single factor experiments.Then the process parameters were optimized by the response surface optimization method.Conclusion:The optimal extraction conditions are water bath temperature 64°C,time 28 min,solid-liquid ratio 1:32,ethanol concentration 66%,under this condition,the maximum extraction rate of chlorogenic acid is 3.058%.After 3verification experiments,the actual extraction rate was 3.041%,which was higher than the 2.8868%highest extraction rate in the single factor experiment,thus confirming that this method has a certain optimization effect.A model of cadmium exposure in rats was used to investigate the protective effect of chlorogenic acid on the intestinal barrier and the role and mechanism of limiting cadmium uptake.32 rats were divided into 4 groups according to the principle of random distribution,pre-feeding for one week,and each group of rats were administered intragastrically one week later.Control group?control,CON?,only distilled distilled water;cadmium injury model group?CdCl₂,Cd?,intragastric administration of 6 mg/kg BW CdCl₂;cadmium injury model+chlorogenic acid treatment group?Cd+CGA?,Gavage 6 mg/kg BW CdCl₂+50 mg/kg BW CGA;cadmium injury model+sunflower seed extract?Cd+SSE?,6 mg/kg BW CdCl₂+50mg/kg BW CGA.The results showed that chlorogenic acid can not only improve intestinal mucosal damage,increase villus height and crypt depth in cadmium exposed rats,but also increase the weight gain and feed intake of cadmium-exposed rats to some extent,and relieve the imbalance of organ index.Can significantly inhibit the cadmium caused by a large number of ALT and AST synthesis,protect the liver function of rats,reduce the toxicity of cadmium.The mechanism is that chlorogenic acid can enhance the intestinal antioxidant function of cadmium exposed rats,reduce intestinal inflammation,reduce intestinal permeability,and limit cadmium by reducing the expression of metal transporters and improving intestinal barrier damage.Absorb,reduce the damage of cadmium on the body.