Exploration Of Nanozyme-based Nanoplasmomic Catalytic Sensing

Since the 1990s,with the development of nanostructures,nanotechnology and nanoparticle,nanoplasma has been widely used in the field of sensors.Nanoplasmonic sensors use localized surface nanoplasma resonance to enhance sensor signals and convert biochemical signals into electrical signals.Because of its high sensitivity,no marking,real-time on-line detection,strong anti-interference ability and no damage inspection,it is widely used in biology,chemistry,medicine,food,and environment.Nanozymes are man-made enzymes that mimic biological enzymes in the natural system,based on the catalytic function possessed by nanomaterials with a diameter of less than 100 nm or functional groups present on the surrounding monolayers.With the characteristics of high catalytic efficiency,stability,economy,and large-scale preparation,it has been widely used in the fields of medicine,food,chemical engineering,agriculture,and the environment.First,a nanoplasma sensor was constructed in combination with the localized surface plasmon resonance?LSPR?phenomenon of gold nanoparticles and simulated glucose oxidase activity.The size and concentration of AuNPs in the nano-plasma sensing system were investigated,and the temperature and pH of the sensing conditions were optimized.The optimized sensor is used for the detection of glucose,and selective and interference tests are performed.The uric acid and urea were selected in the interference test.The experimental results showed that the nano-plasma sensor has good selectivity and anti-interference ability to urea,while uric acid interferes with the sensor.The sensor has the advantages of higher sensitivity and lower detection limit.Due to the use of dispersed nanozymes,the stability is poor and the consumption is high.Secondly,we have found that uric acid can be used to detect uric acid using glucose nanoplasma sensing because of its strong interference with nanoplasma sensors.The colorimetric test was used to further confirm the interference with uric acid,and the uric acid was detected.The colorimetric method for detecting uric acid was compared with the nanoplasma sensing method.This detection method needs to be further improved and in-depth.Finally,for the stability and high consumption of nano-plasma sensors,the optimization and improvement are further improved.The main tasks include the preparation and characterization of AuNPs@SiO₂ composite nanomaterials,the optimization of pH and temperature for sensing conditions,and the detection of glucose.The test successfully solved the problem of unstable instability and high consumption of the dispersion,and at the same time ensured the complete resonance absorption peak of the small-sized nano-gold?3 nm?in the micro-spectrometer and did not generate a large amount of aggregation during the high-temperature treatment.