The Processing Technology Standardization And Quality Standard Research Of Bombyx Batryticatus Powder

Bombyx Batryticatus(BB)is dry silkworm,which is the 4 to 5 instar,infected with Beauveria bassiana artificially?It has the pesticide effects of dispeling wind and relieving spasm,dispelling wind and relieving pain,and eliminating the phlegm.“Lei Gong processing theory”recorded Bombyx Batryticatus powder decoction pieces,“you have to immerse Bombyx Batryticatus in rice water,when silkworm salivate is liked snail mucus and appeared,Bombyx Batryticatus is floating on the rice water,then they were filtered off,dryed by slight fire,cleaned the yellow meat hair of silkworm by cloth,black mouth carapace is removed,Bombyx Batryticatus is pounded and sized?”Through literature research,it was found that the processing method of Bombyx bassiana evolved from rice rinsing to bran frying,although the methods were different,they were generally able to deodorize and reduce dryness.The processing method of Bombyx bassiana powder(Lei Gong processing theory)has distinctive characteristics.However,the current version of Pharmacopoeia of people's Republic of China and local processing standards has not embodied Bombyx bassiana powder,The processing technology of Bombyx Batryticatus powder has not been reported yet.In addition,the mechanism of anticonvulsant action is not clear.In view of the above problems,this paper studies from the following aspects.Study on anticonvulsant effect of Bombyx Batryticatus powder,In this paper,the anticonvulsant effect of Bombyx bassiana powder was studied by maximum electroshock model,pentylenetetrazol induced metrazol seizure test and kindling model of epilepsy chronically induced by pentetrazol.The latency time of clonic convulsion,the latency time of tonic convulsion and the number of mice have been observed for 30 min.The rate of tonic convulsion was calculated.Amino acid neurotransmitters(glutamate Glu and gamma-aminobutyric acid GABA),which are closely related to the pathogenesis of anticonvulsion,were detected by HPLC-DAD method.The results showed that the Bombyx Batryticatus powder had a good anticonvulsant effect on the above three models,and the mechanism of its anticonvulsant action was also discussed.The research of Bombyx Batryticatus powder processing technology standardization.Through using orthogonal design method,this subject took rice water rate?material to liquid ratio?bleaching time and temperature as factors.It took the comprehensive score of the external odor evaluation and the determination of the extract content as the evaluation index,The optimum processing parameters were determined as follows: the ratio of rice water was 1: 5,the bleaching time was 24 h,and the bleaching temperature was 30 ?.Study on quality standard of Bombyx Batryticatus powder.In this paper,the relative researches of quality standard for Bombyx Batryticatus powder were studied,including identification(microscopic,TLC),inspection(impurity,moisture,total ash and acid insoluble ash),extract,content determination(protein content,beauvericin,ammonium oxalate)and HPLC fingerprint.The identification of microscopic method and a TLC method for amino acids of Bombyx Batryticatus powder was established,The inspection,extraction and content determination were established,the limits and standards were worked out.In summary,this subject provides scientific basis for the establishment of quality system of Bombyx Batryticatus powder,through the study of anticonvulsant effect,processing technology and quality standard of Bombyx Batryticatus powder.It also lays a foundation for the establishment of anticonvulsant mechanism and evaluation criteria of Bombyx Batryticatus powder.