Research On Preparation Technology And Quality Evaluation Of Eucommia And Salt Eucommia Standard Pieces

The preparation of trditional chnese mdicine decoction pieces is based on the specific method of processing drugs,in a similar way,the evaluation of the quality of traditional chinese medicine decoction pieces is based on its own processing degree.Traditional chinese medicine decoction pieces can give more expression to its integrity and specificity than the single chemical reference substance.Adding traditional chinese medicine decoction pieces to the research of traditional chinese medicine standard material has it's practical value.The experiment choosed Eucommia ulmoides as the object of the study,established the preparation technology and quality evaluation of Eucommia and salt Eucommia standard pieces,and explored how to use the contrapositive pieces to the quality control of traditional chinese medicine.Before the experiment,the research group gave investigated the genuine producing area,made the specification origin of Eucommia ulmoides and collected the high quality medicinal materials from Wangchang county of Sichuan,than entrusted the worker who has the production and processing experience more than 15 years to process the medicinal materials to be Eucommia ulmoides and salt Eucommia pieces in Sichuan Chinese medicine yinpian Co·Ltd.At the same time,we also collected the pieces of different batches from two mainstream companies on the market.The main research content of the paper as follow:1 Established the grinding technology of Eucommia ulmoides and salt Eucommia piecesEucommia ulmoides is hard to crush entirely because ofthe silk in it,which has influence on homogeneity of it's powder.According to microexamination and particle size distributionof Eucommia and salt Eucommia pieces in different grinding manners and the particle size distribution of Eucommia reference drug,ultrafine grinding is a optimum grinding manner of Eucommia and salt Eucommia pieces.By means ofspectral differences,color and internal content which can evaluate the homogeneity of Eucommia and salt Eucommia pieces,ultrafine grinding is the besttechnology,and ensure its powder which can through the 150 mesh number should not be less than 74%?65%,all of them can through the NO.2 screen.2 Research on quality standard of the contrapositive pieces and quality analysis of the saled piecesAnalyzed all of pieces and added TLC and the determination of acid-insoluble ash based on normal analysis method from Chinese pharmacopoeia of 2015,and optimize the determination methods,established quality standard of the Eucommia ulmoides and salt Eucommia contrapositivepieces.3 Study of characteristic spectrum of contrapositive piecesThe HPLC characteristics of Eucommia ulmoides and salt Eucommia contrapositive pieces had established and compared it with the original medicinal materials,the original pieces and the saled pieces,we found that there was no significant difference in the HPLC chromatogram between the contrapositive pieces and the original pieces,which indicated that the homogenization process of the contrapositive pieces did not change the chemical properties of the pieces.The contrapositive slices prepared under the process could be used for quality control of Pieces.There was no significant difference in the HPLC chromatogram between the contrapositive pieces and the saled Pieces of Eucommia in the consistency evaluation,which indicated that the contrapositive pieces of Eucommia had a wide range of applicability and could be used for the quality control of Eucommia pieces,but there has significant difference in the HPLC chromatogram between the different batches of saled pieces of salt Eucommia,the results showed that the chemical composition and content of Eucommia were changed with the increase of processing ability,which resulted in the difference of HPLC chromatogram.To solve this problem,we should establish a reasonable processing technology,and unified salt Eucommia processing standards.4 Study of stabilityThrough the influencing factors,it was found that the contrapositive pieces of Eucommia and salt Eucommia had hygroscopicity and should be kept in dry place.The content of pinoresinol-di-O-b-glucopyranoside in Eucommia and salt Eucommia was slightly decreased under strong light or high temperature condition.Refer to the manner of packing of Eucommia reference drug,thecontrapositive pieces of Eucommia and salt Eucommia should be sealed with brown bottle,and 1g per bottle for 6 months.Accelerated test result shows that the effective period of Eucommia and salt Eucommia is 2 years.