Study On Potential Toxic Mechanism And Rapid Warning Detection Method Of Several Furocoumarins Condiment Molecules

In order to study the potential toxicity of xanthotoxin, bergapten, xanthotoxol whichare three furocoumarin flavoring agent molecules, this paper will start from several aspectsas follows. Firstly, resonance light scattering is used to explore the ability of xanthotoxin,bergapten and xanthotoxol to enter the E.coli cells and combine with its DNA. Secondly,resonance light scattering spectrometry, UV spectrophotometry, DNA thermaldenaturation experiments, viscosity test and agarose gel electrophoresis are used to studythe interaction between the three molecules and DNA. We compare with these fivemethods and select the method which is more efficient. Then an easier method is selectedto detect and calculate the the DNA saturation binding value with xanthotoxin, bergaptenand xanthotoxol, and compare with the saturation values of ethidium bromide andisopsoralen. And a new method to quickly evaluate the potential toxicity of some planefurocoumarin molecules is built by the DNA saturation binding value. Finally, theinfluence of environmental factors on this interaction is investigated. The researchachievements are mainly as followed:1. Resonance light scattering is slected to explore the ability of xanthotoxin,bergapten and xanthotoxol to enter the cells. And the result shows that it is not varygreatly in their abilities to enter the cells. When the reaction time reaches8.5hours, theresonance light scattering signal for the bergapten and xanthotoxol groups are nearly thesame, followed by the xanthotoxin group, which indicates that the abilities of bergaptenand xanthotoxol to enter the cells are a little higher than xanthotoxin group.2. Resonance light scattering spectrometry, UV spectrophotometry, DNA thermaldenaturation experiments, viscosity test and agarose gel electrophoresis are used to studythe interaction between the three molecules and DNA. The results of agarose gelelectrophoresis shows that these three molecules interact with DNA indeed. All ofresonance light scattering spectrometry, UV spectrophotometry, DNA thermaldenaturation experiments and viscosity tests show that these three molecules may combinewith DNA by intercalation. Furthermore, the result shows that resonance light scattering ismost efficient way of the five methods.3. In a neutral environment, the DNA saturation binding value of xanthotoxin,bergapten, xanthotoxol are0.18,0.18and0.24, and the saturation values for ethidiumbromide and isopsoralen are14.70and0.34, respectively. As a consequence, it appearsthat the potential toxicity of xanthotoxol is a little higher than that of xanthotoxin,bergapten. However, all of these three molecules potential toxicity are a little lower than isopsoralen and is much lower than that of ethidium bromide. By this way, a new way toquickly detect the toxicity of some furocoumarin molecules is established.4. Also, resonance light scattering spectrometry is slected to investigate the influenceof environmental factors on the DNA saturation binding value of xanthotoxin, bergapten,xanthotoxol. The combination capacities of DNA and xanthotoxin, bergapten, xanthotoxolchange when the environmental conditions and other factors change. An acid environment,an alkaline environment, glucose solution and low concentration of Zn2+can reducepotential toxicity of xanthotoxin, and sodium chloride, glutamic acid, vitamin C andL-proline solution can increase it potential toxicity; glucose solution and lowconcentration of Zn2+can reduce potential toxicity of bergapten, and an acid environment,sodium chloride, L-proline and vitamin C solution can increase it potential toxicity, analkaline environment and threonine solution have no obvious effects on it potentialtoxicity; glucose solution and low concentration of Zn2+can reduce potential toxicity ofxanthotoxol, and an acid environment, sodium chloride, L-proline and vitamin C solutioncan increase it potential toxicity, an alkaline environment and threonine solution have noobvious effects on it potential toxicity.