| "Chronic bones disease"which includes the spine and extremities bone and joint degenerative disease,is an important part of the worldwide attention"chronic disease prevention and control”system,and also a high incidence of complex disease in an aging society,it is of serious harm to health and easy to relapse.Western medicine for the treatment of this disease is easy to cause relatively serious ADR,and surgical treatment is tending to recrudesce,nevertheless traditional chinese medicine can be used for nursing patient's health or long-term treatment.This feature along with the deepgoing fluence of Chinese medicine culture on our countrymen give Chinese medicine for this disease a huge market and this market demand will be continued with the improving of living standard and the health care system.The subject makes“Jingu tablet prescription”as the research object,The prescription composed by ten kinds of traditional Chinese medicine including eucommia bark,radix dipsaci,lycopodium clavatum,inherited,etcThe viewpoint of traditional works"Qian Jin Fang","Shang Han Lun":“dispel wind-cold,nourishing the liver and kidney,strong bones and muscles”was inherited,the“choroids theory”and modern research literature theory was introducted,and the dffects of"reunion of fractured tendons and bones,conserve blood vesse,relieve pain and inflammation were increased.Preparation process study:process parameters of water extraction,alcohol extraction and drying were determined by L9(3~4)orthogonal test.i.e,(1)Water extraction was indexed by pinoresinol glucoside content,and the alcohol extraction was indexed by asperosaponin VI content.2 hours,11 times water and alcohol for the first extraction;three times,1.5 hours,9 times water and alcohol for the second and there time respective.(2)the extracts were concentrated,dried and the final relative density was 1.12±0.05(60~oC),with 182 ~oC for the air-in temperature,and 90 ~oC for air-out temperature;the parameters were verified by optimizing test.At the same time,three batches of pilot trial study were carried out,which was proved reasonable and feasible on technological conditions,easy to operate and will be of guiding significance for industrial production.Quality standard study:TLC,heavy metal and arsenic salt check,microbial detection method research were carried out in this paper.The method for determination of content of teasel saponin VI was examined by HPLC and showed a good linear relationship within the scope of 360.8-3608.0 ng.The regression equation was:lny=1.6007lnx+2.8407,r~2=0.9996.According to the quality standard,the accelerated stability test and other related indicators detection of the pilot were also completed and the results showed that all the indexes met the requirements,and the stability was good in six months.UPLC/Q-TOF-MS/MS study:UPLC/Q-TOF MS/MS positive and negative ion full scan mass chromatograms were established in this paper.Compounds'molecular formulas were determined according to the information of control article,molecular ion accurate mass,isotopic quasi fitting degree analysis and matching formular search in the database,so that the various peaks were identified,and assigned to the source of chromatographic peaks.Results:38 peaks were identified from the chromatogram of JinGu Tablet.This study clarified the chemical composition more comprehensive,set a certain foundation of the studies of pharmacodynamic material basis,quality control and the authenticity identification. |
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